细胞核荧光探针的设计合成及应用-精细化工专业论文.docx

细胞核荧光探针的设计合成及应用TheSynthesisandApplicationofNucleus—TargetableFluorescentProbesAbstractBio—technologieswhichbasedonfluorescentprobesaremoreandmoresignificantinlifesciencefield．Wim也erapiddevelopmentofbio-imagingtechnologies，fluorescentprobehasalreadybecametheimportanttooltostudythemedicineandbiologyissues．Tothisdate，mostresearchersconcentratedtheirstudiesonthefluorescentprobes，whicharetargetabletothesubcellularstructureandcangivethequantitativeinformation．NucleusiSthecellcontrol hub．whichplaysanirreplaceableroleincellmetabolism，growth，differentiation，andotherprocesses．AlthoughquiteafewfluorescentprobeshavebeenreportedtoshowhighaffinitytoDNAinbuffer，onlyaverylimitednumberofthemCanstainthenucleusspecificallyinlivingcells．Sothedevelopmentofanucleus．targetablefluorescentprobe，whichhasaspecificfunctionisveryimportant．Inthispaper，basedoncommercialHoechstdye，wedesignedandsynthesizedthreefictionalizednucleus．targetablefluorescentprobes，andpreliminaryevaluatedtheirpropertiesandapplicationsthroughpreformingsomeexperiments．WehavedevelopedprobeHoe-Nl，by‘Click’connectionofaHoechstunitandanaphthalimidedye．Thisprobeemitsdualfluorescencebandscenteredat450nlnand505nln．Inbuffersolmion．Hoe．NlshowssensitiveratiometricdeteetionofDNAconcentration，andisusedfortheevaluationofDNAdamagebyhydroxylradicals．Hoe—Nlalsoexhibitsthespecificallyandwash．freestainingoflivingnucleusinallthetestedcells，includingL02，SMMC．7721，COS一7，MCF一7，Hela，raw246．7cells．Mostimportantly，Hoe—Nl，iSfirstprobeapplicablefortwo．channelratioimagingofnucleusDNAcontentinlivingcells，whichhasbeendemonstratedbythevisualizationandthequantitativeanalysisofnucleusDNAdamageinducedbyhydroxylradical．O．phenylenediamine(OPD)iSknowntoreactwithnitricoxide烈O)togenerateatriazolestructure．Impairedfrom也is，wedesignedanucleus—targetablefluorescentprobeHoe．N0basedoncommercialHoechstdye．ProbeHoe—NOCaninteract withDNAinbuffersolution．whichindicatedthatitCanbetargetedintonucleus．TheNOtitrationexperimentwasperformedandthehigh．resolutionmassspectroscopy(