稀土上转换发光纳米材料用于小动物淋巴系统成像的研究-无机化学专业论文.docxVIP

复 旦 大 学 2 00 8 级 硕 士 学 位 论 文 A b s tra c tA b s tr a c tL y m phog rap hy is o ften u sed fo r th e d iagn osis o f ly m ph atic m etastasis in o nco lo gy . F luo rescent b io im ag in g is a un iq ue ap pro ach to visualize th e deta ils in celland an im al m o d els in v iv o . It is a challen g e to im pro ve sig nal-to -n o ise ratio an d lig ht p enetratio nin fluorescent bioim aging. T m 3+-doped rare-earth upconversion nanophosphors(U C N P s) u nd e r co ntin u o u s-w av e (C W ) exc itatio n at 9 80 n m d isp lay u n iq ue u pcon version lum in escence p eaked at 80 0 n m , and beco m in g o n e of the m o st p ro m isin g c lasses o f lu m in escent lab e ls. T h is thesis is fo cu sed o n th e app licatio n o f U C N P s in ly m p hatic im ag e an d com po sed o f tw o parts.I,U p co n v ersio n n a n o p h osp h o rs fo r th e ly m p h n od e im a g e in vivo .U sin g U C N P s as lu m in escent p rob es, m u ltip ly d ra in ag e ly m p h n o d es fro md ifferen t ly m p h atic b a sin s in an im al m o d e ls (n ude m o u se , K u n -m in g m o u se, rats, rab b it) h ave b een v isua lized rea l-tim e in vivo . U C N P s p ro v id es g reat ten d en cy to w ard ly m p h sy stem w ith h ig h sign al-to -n o ise ratio . F u rth erm o re, w ater-so lu b le n an o p ho sph o rs w ith size of 2 0? 4 2 n m is ind icated as th e optim ized can d idate fo r ly m p h atic im ag e .II. H y a lu ro n ic a cid co a ted u p co n v ersio n n a n o p h o sp h o rs fo r th e ly m p h a tic v esselta rg et im a g e in vivo .H y a lu ro n ic ac id (H A ) co ated u p co n v ersio n n ano p h o sph o rs (H A -U C N P s) hav e b een sy nth esized th ro ugh e lectro static interactio n . T he as-p rep ared H A -U C N P s are w e ll d isp ersed in w ater an d h av e ex cellen t p h o to stab ility . B ecau se o f th e sp ec ial affin ity betw een H A an d ly m p h atic en d othelia lcells hy aluro n ic acid recepto r-1 (