用灰思维建立中药半仿生提取研究的技术平台-中药学专业论文.docx

Pretendingtobethedregsof5molecularw eight三1000Daextractionmethodextractsthemaineffieacyand肠xicity;⑥ on尺hizomaC》少eribysFE一eo:extr actionresi如e雄ersmolecular weight三1OOODa extractand rabbitserum with HPLC fingerp rintforComparison:⑦extraetionmethodsonthefivekinds of molecularweight三l000Daextraet ofrabbit eoniaining rabbitintestinalsm ooth m uscle for the role饰ofeomp如son;⑧ comprehensive evaluation ofthe final，optimized 尺hl.zoma o，eriSF E一COZextraetionresidueafterthebestm ethodsandProcessconditions.Results:①Rhizomao夕erimeet the”ehinesepharm aeopoeia，，underthe2005version ofone ofthere明irementsfor明alified slices;② preparation ofoptimizedmolecularweight三1000DaSBE extraeteonditions\were:3 创ed waterPH were:2.0，6.5，9.0 :extraetion time were:2.0，1.0，1.o h:③ optimized semi一ionie extractionoPtimaleoneentrationof60% ethanolPreeiPitation，thebestethanoleoneentrationof55% ;④Rhl.zoma自了，eribytheresidueafterSFE一CO:5molecularweight 三10o0Damethodextractsthe valu e ofthe eom Prehensive evaluation index order:Y sBE Y A EY w E Y sBAEY、E;⑤mainpharm aeod”amieresultsshow thatthemolecula:weightof三1oooDasw aysto extraet，SB E m ethod to the m ostPotentefficaey;aeutetoxieitytestshow ed th atthemoleeularweight三x000Da5wa”toextraetthebasiedrug一free:⑥Rhl’zoma0少eriresidueafterbySFE一CO:5ways toextraetand them oleeularweightof生1000Daseru们nw ith H PLC fingerp rinteom Parison show sthat eharaeteristiePeak softhetotalareato thel哗est缈up ofsBE solution;⑦ 5 DifferentM ethods允rthemolecularweight of三1000D a extractseontaining serum to serum eo ntain in g SB E m ethod intestine in vitroinh ibition ofthe strongest;⑧ comprehensive evaluation :esultsshow that，尺人抢omaC叩eribytheresidueafterSFE一CO:extraetion15bettertoSBE.Conclusion:FortheextraetionofRhl’zoma0 夕eri，thefirsttoSFE一COZextraetionofvolatile com Pounds，residue after extraetion SB E then ex tracted by eentrifugation -m embrane filtration m ethod extraets a m olecularweight 二