超顺磁性Fe3O4纳米粒的制备及其对小鼠的急性.docVIP

论著 超顺磁性Fe3O4纳米粒对小鼠急性毒作用 张玉坤，罗聪，（重庆医科大学附属儿童医院【摘要】目的：制超顺磁性Fe3O4纳米粒（superparamagnetic Fe3O4 nanoparticle对小鼠的急性毒性作用。方法：采用化学共沉淀法制备，用透射电镜、Zeta 电位分析仪和振动样品磁场计等形态观察结构表征。60只小鼠按给药随机分为（n=20）口服灌胃组总剂量2104.8mg/kg、容积ml/kg），腹腔注射组总剂量mg/kg、容积ml/kg），尾静脉注射组总剂量438.5mg/kg、容积10ml/kg）;每组另10只小鼠予生理盐水作为对照观察小鼠的一般情况检测小鼠主要血生化指标；HE染色观察主要脏器的病改变瑞染色观察骨髓。结果：粒径大小为15-20 nm, 饱和磁化强度为21.431emu/g,剩余磁化强度为0.1016emu/g。小鼠均未死亡血生化指标无显著差异和骨髓均未见炎症水肿、变性、坏死等改变。结论：超顺磁性Fe3O4纳米粒超顺磁性Fe3O4纳米粒对小鼠无明显急性毒载附药物。 关键词：超顺磁性纳米粒；急性毒性；Preparation of Superparamagnetic Fe3O4 nanoparticles and their acute toxicity on mice ZHAN Yu-kun, LUO Cong, ZHU Chao-min,( Childrens Hospital of Chongqing Medical University, 400014) [ABSTRACT] Objective: To prepare superparamagnetic Fe3O4 nanoparticles (SPFN) and to study their acute toxicity on mice. Methods: The SPFNs were obtained by means of chemical coprecipitation,and their morphology and structural characterization were observed and determined by transmission electron microscope, Zeta potential analyzer, and vibratory sample magnetometer, etc. According to different ways and dosage of the drug 60 mice were randomly divided into oral administration (with a total dose of 2000 mg/ kg and a volume of 25 ml/ kg, n = 20), intraperitoneal injection (with a total dose of 1625 mg/ kg and a volume of 20 ml/ kg, n = 20) and caudal vein injection (with a total dose of 438. 5 mg/ kg and a volume of 10 ml/ kg, n = 20) groups. Another 10 mice in each group receiving the same dose of normal saline via the same pathway served as the controls ( n = 10). The general condition of mice was observed during the experiment, the major blood biochemical indicators were analysed by the automatic biochemical analyzer, and the pathological changes of major organs and the myeloid tissue were observed by H-E staining and Wright staining respectively. Results: We have prepared SPFNs with the particle diameter 15-20 nm , a saturated magnetization of 21.431 emu/g, and a remanent magnetization of 0.1016 emu/g. There was no dea