通过全基因组扫描研究定位小鼠性发育相关基因-应用化学专业论文.docx

通过全基因组扫描研究定位小鼠性发育相关基因中发现:正交FZ代小鼠中，5号和12号染色体上各有一个区段提示与性状相关(DSMit367，LOD=2.423;D12Mitl44，LOn=2.196)，反交FZ代小鼠中没有阳性结果。并应用连锁不平衡分析验证了这两个位点与小鼠性发育启动天数显著相关(P0.05)。通过本实验，使我们对小鼠全基因组中与性发育相关的位点有了更全面的了解，同时为下一步缩小相关区段，进行精细定位奠定了基础。此项研究最终也将为从基因水平解释调控哺乳动物性发育启动的分子机制打下基础。关键词:小鼠模型，性发育启动，短串联重复序列，单核甘酸多态，数量性状基因座位，基因分型通过全基因组扫描研究定位小鼠性发育相关基因LOCALIZATION OFQTLSREGULATINGTHEONSETOFPUBERTY BY WH O LE G EN O M E SCANN INGINM ICEABSTRA CTP uberty，ofteninflueneedbybothenvironmentaland gen etiefactors，15a comP lieated biologie Processinvolving sexualm aturation and gainingabilityofreProduction.hiordertofindtheQTLsregulatingtheonsetofPuberty，twoinbredstrainsofmieeC3H/HeJand C57BL/6JwereselectedandtwokindsofFZPedigreeswerefoundedbyhybridizationofthetwostrainsinreeiProealcrosses.Balano一PrePutialseParation(BPS)wasusedtoeharacterizethePubertalonsetofmalemiceandvaginaloPening(VO)wasused toehara cterizethePubertalonsetofthefem ale.An d theageatPubertalonsetofallthesenilee(Pareni，FIand FZ)werereeorded.And thePhenotyPeofPubertaltimingofallthemice印arent，FIand FZ)wererecorded.Th evarianee forPubertaltiming betw een Progenitorstrains，Fland FZ Progenyw ereanalyzed.PhenotyP iedatashow ed thatinfem alem iee，signifieantdifference ofthistraitwas observed betweenB6andC3H(P=3.7xlo一‘3) as wellas betweendireet andreeiProeal F一hybrids(P=5.4x10一，)，butnotbetweendireetandreeiProealFZhyb五ds(P=0.0941).An d infemalemiee，theheritabilityofthetiming ofPubertyindirectandreeiProeal erosses 15 63.97% and 68.48%，resPectively.The resultdemo nstratedthatwehadsetuP twosueeessfulPedigrees.七通过全基因组扫描研究定位小鼠性发育相关基因259PhenotyPicallyextremeFZ femalemieewith6~ 10PuP Perlitterfrom 534 femaleFZProgeny wereseleeted and ehromoso力以e-widescreenwasca币edoutonwh olegenome.86STR andSNP markerswerechosenwithanaverageintermarkerdistaneeofabout15toZOeMforthePositionaleloningofQTLsregulatingtheonsetofPuberty.Previous studies ofourresearch grouP loeated thr ee QTLs 廿iroughchromosomalseanning oneandidateehromosom es6，13andX.hithedireeterossPoPulation