poster讲课幻灯片.ppt

Made by Group2 We divided our article into six section, including abstract, introduction, methods, discussion and reference. According to six section, we will introduce our poster completely . Conclusion: In this study we report the water stress induced protein aggregation and its abrogation followed by expression of group 1 and group 2 LEA proteins of water soluble proteomes in horsegram Discussion:The role of LEA proteins in relation to protein aggregation during water stressed conditions was discussed Result: We found no cross reaction of LEA 1 with proteome of 48 h stressed embryonic axis and 72 h stressed root and shoot samples. However, LEA 2 antibodies were cross reacted with four polypeptides with different molecular mass in shoot tissue samples and found no reaction with root proteome as evidenced from immuno-blot analysis. Method: Tissue-speci?c expression of LEA 1 protein decreased in the embryonic axis when compared to cotyledons in 24 h stressed seedlings. Abstract Introduction Method and materials 1. Plant material and stress treatments 2. Relative water content 3. Protein extraction and estimation 4. Protein aggregation 5. Electrophoresis 6. Protein gel blot analy Result 1. Expression of group 1 LEA proteins 2.Expression of group 2 LEA proteins Discussion In summary, we presented data on the developmentally regulated tissue speci?c expression of LEA 1 and 2 proteins under different levels of water stress in horsegram. From these results we hypothesize that the reduced protein aggregation and water stress induced accumulation of proteins could perhaps be due to high expression of LEA proteins particularly LEA 1 and 2 which are t