添加菊科植物黄花蒿提取物对奶山羊乳中cla含量影响及机理研究-动物营养与饲料科学专业论文.docx

The Study on Effects of Compositae Artemisia annua Extracts onCLA Content in Goat Milk And the Relative MechanismAbst r actThe thesis studied exlraction technique for Artemisia annua in orthogonal experiment design and its active components by GC-MS and LC-MS，and measu red effects of the Artemisia annua extracts on C18 UFA biohydrogenation rate in vitro，VFA in the rumen，VA and CLA in the rumen，blood plasma and mi lk in dairy goats，artery-venous fatty acids composition in the mammaηI glands，SCD in mammary glan ds in vivo and fatty acidscomposition in mam mary glands cell s in vitro. A preliminary study on the effects of A rtem i sia annua extraction on CLA i n goats milk and its mechan i sm was canied .The thesis is composed of five parts as followsPart 1 Screening extraction technique for Artemisia annua and analysis of its activecomponentsAccording to extracting rate，an extraction techniqlle for Artemisia annlla in orthogonal experiment design was screened and its active components were analysed through GC-MS and LC-MS method . The results showed : (?)The parameters for a proper extraction tech n ique were 55% ethanol concentration，95C extraction temperatu re and 2 h extraction time;② The active components were sesquiterpenoi巾，aromatics，fa时 acids， steroids，flavonoi 巾，triterpenoids，aft， alkaloids，phenols，heterocycle compounds ，terpenoids and other compounds through GC-MS method and LC-MS method .Part 2 Studying the effects of Artemisia annua extracts on VA，CLA，C18 UFA biohydrogenat ion rate and other fatty acids concentrations in vitroDoses of Altemisia annua extracts for supplementation as freeze-dri巳d basi s were :O(as conlrol)，3，30，300 and 3000 mg!L mixed culture fluid respectivel y in vitro.The resulls showed ①CI8 UFA ( c9CJ8:l 、CJ8:2 和 C 18:3) biohydrogenat ion rate increasedgradllally with clllture time and arrived the highest vallle at 24h ，C18 2 biohydrogenation rate was 68.33-82.71% at 24