intratracheally applied rsp-c surfactant exhibits no anaphylactic shock reactions in a guinea pig model of acute lung hypersensitivity：气管内应用rsp-c表面活性剂具有无过敏性休克反应在急性肺过敏豚鼠模型.Image.Marked.pdfVIP

Exp Toxic Patho! 1999; 51: 495-505 URBAN FISCHER http://www.urbanfischer.de/jouma!s/exptoxpath lInstitute of Pathology and Toxicology, Byk Gulden, Hamburg, Germany Intratracheally applied rSP-C surfactant exhibits no anaphylactic shock reactions in a guinea pig model of acute lung hypersensitivity 1 2 PAUL-GEORG GERMANN • , JORG KEMKOWSKI!, DIETER HAUSCHKE!, WOLFRAM STEINHILBER!, and DIETRICH HAFNERI With 12 figures and 4 tables Received: December 6, 1998; Accepted: December 21, 1998 Address for correspondence: 2Dr. PAUL G. GERMANN, Section Head of Pathology, Preclinical Safety, WSH-288l.4.07, CH - 4002 Basel, Switzerland. Key words: Ovalbumin; Lung hypersensitivity; rSP-C surfactant; Immunohistology of SP-C; Shock anaphylactic Sur- factant, rSP-C; Hypersensitivit, lung; Anaphylactic lung shock. Summary bronchus-associated infiltration with eosinophilic granulo- cytes and in the formation of peripheral emphysema, but with The effect of the intratracheal administration of the re- no dose-dependancy. A slight dose-dependent increase in combinant SP-C surfactant apoprotein (rSP-C) with phos- the deposition of peribronchiolar eosinophilic foreign mate- pholipids (PL) in comparison to an ovalbumin induced ana- rial was evident. In contrast to this, the number oflipid-loaden phylactic shock reaction was studied in guinea pigs lungs. alveolar macrophages seemed to decrease with increasing doses of rSP-CIPL. The immunohistological investigation Narcotized guinea pigs were challenged by intratracheal administration on