本科毕业论文（设计）--盐藻SOD分子特性研究.docVIP

本 科 毕 业 设 计 (论 文) 盐藻SOD分子特性研究 Study molecular properties of SOD from Dunaliella salina 学 院： 专业班级： 学生姓名： 学 号： 指导教师： 2010年6月 连云港 毕业设计（论文）中文摘要 盐藻SOD分子特性研究 摘 要：超氧化物歧化酶是生物体防御氧化损伤的一种主要酶在临床治疗和预防、食品、化妆品等领域应用广泛。本实验对盐藻SOD的进行了。加入-3570℃水浴条件下热处理10min，SDS-聚丙烯酰胺凝胶电泳，检测出SOD谱带，与标准蛋白比对得知，其分子量为23.1KD。H2O2，明显发现样液中SOD活性受到抑制。结合现有资料判断确认，盐藻中的SOD应为Cu/Zn-SOD类型。 关键词：SOD；盐藻；分子特性 毕业设计（论文）外文摘要 Study of molecular properties of SOD from Dunaliella salina Abstract: Superoxide dismutase is the organism oxidative damage defense of one of the main enzyme. In clinical treatment and prevention, food, cosmetics and other fields are widely used. In this experiment, the molecular properties of SOD Dunaliella salina was studied. Experimental training to take the logarithm period, the cell through repeated freeze-thaw and ultrasonic broken obtain SOD enzyme, join - 35 degrees of acetone precipitation were at 70 degrees heat water purification 10min, under the condition of 11.30 multiples. The purified enzyme liquid through SDS- polyacrylamide gel electrophoresis, detection a SOD spectrum, and the standard that the molecular weight than protein for 23.1 KD. Through the spectrum of purification of SOD, detect the ultraviolet absorption wavelength 259nm for maximum absorption peaks, visible wavelength area biggest for 671nm. By adding different concentrations of H2O2, obviously found samples of suppressing SOD activity. Combine existing material, the judge should Dunaliella salina SOD was Cu/Zn - SOD type. Keywords: SOD；Dunaliella salina；molecular properties  目 录 1 绪论………………………………………………………………………………1 1.1 盐藻的简介……………………………………………………………………1 1.2 杜氏藻开发应用的现状和前景……………………………………………2 1.3 超氧化物歧化酶(SOD)的概况……………………………………………3 1.4 植物SOD研究…………………………………………………………………3 1.5 本课题研究的意义……………………………………………………………7 2 材料与方法 ……………………………………………………………………7 2.1 实验材料………………………………………………………………………7 2.2 实