毕业论文：The construction of fas III prokaryotic expression vectors.docxVIP

The construction of fas III prokaryotic expression vectors Abstract: Drosophila Fas gene is protein coding gene in testis, mainly expressed in hub cell. In this experiment, by means of gene cloning technique, the target gene FasIII was inserted into the plasmid vector PET28a which was digested by two enzymes of BamHI and HindⅢ. so as to construct the prokaryotic expression vector PET28a-fasⅢ, then the recombinant plasmid was transformed into Escherichia coli TOP10, achieve Fas III gene prokaryotic expression. The plasmid vector was marked with kanamycin resistant (Kanr) , Only was transfor