06474174手机 电化学检测.pdfVIP

A HANDHELD, CELL PHONE-BASED ELECTROCHEMICAL BIODETECTOR 1 2 2 P. B. Lillehoj, M.-C. Huang and C.-M. Ho 1Michigan State University, East Lansing, MI, USA 2University of California, Los Angeles, CA, USA ABSTRACT MATERIALS We present a handheld, biomolecular detector Microfluidic chip based on a cell phone platform. This prototype Silicon and glass wafers (100 mm diameter) integrates a conventional smart phone with a custom were obtained from TechGophers Corp. (Los electrical circuit and a microfluidic chip, which Angeles, CA). PDMS prepolymer and curing agents enables a portable, lightweight biodetection system. (Sylgard 184) were obtained from Dow Corning An electrochemical detection scheme is utilized, (Midland, MI). PEG (MW 200) was obtained from allowing for rapid ( 20 min), high sensitivity, Sigma-Aldrich (St. Louis, MO). Colored dye for analytical measurements. As proof of concept, flow visualization was purchased from a local Plasmodium falciparum histidine-rich protein 2 vendor. KMPR 1050 was obtained from MicroChem (PfHRP2) was detected in buffer solution, with a Corp. (Newton, MA). AZ 4620 photoresist (Shipley, lower limit of detection of 16 ng/mL. Based on its Marlborough, MA), hexamethyldisilazane (HDMS), compact size and