annexin a1和p19在胆囊癌中的表达及其临床意义的分析word格式论文.docxVIP

Western-blot法定量分析30例胆囊癌组织标本及30例胆囊良性组织标本中ANXA1蛋白灰度扫描与内参照β-actin 蛋白灰度扫描相对比值平均值分别为0.7442±0.0528 , 0.1570±0.0326，二组数据经t 检验有统计学差异;两组织中P19 蛋白灰度扫描与内参照 β-actin 蛋白灰度扫描相对比值平均值分别为0.0976±0.0299, 0.7299±0.0874，二组数据经t 检验有统计学差异。结论1.ANXA1 在PCG组织的阳性表达率越高，则肿瘤的恶性程度越高，患者预后相对较差，生存期较短。2.P19 蛋白在PCG 组织中的表达缺失率越高，则肿瘤的恶性程度越高，患者的预后越差，生存期越短。3.ANXA1、P19 蛋白在PCG 组织中表达有明显差异，其阳性表达率与肿瘤的病理分级、临床TNM分期、淋巴结转移密切相关，可作为判断PCG 临床病理发展的参考指标之一。4. ANXA1 及P19蛋白的表达情况均为影响胆囊癌患者预后的独立因素，且二者之间的表达呈负相关，联合检测ANXA1 及P19 蛋白可望成为PCG 早期诊断及预后判断的重要参考指标之一。关键词胆囊癌，胆囊良性组织，ANXA1，P19ExpressionsofAnnexinA1（ANXA1)andP19proteiningallbladdercarcinomaandtheirclinicalsignificancesABSTRACTBackgroundandObjectivePrimarycarcinomaofthegallbladder(PCG)isa commonmalignancyofthebiliarysystem,inrecentyears,theincidencehasincreasedyear byyear.AnnexinA1(ANXA1)washighlyexpressedinavarietyofmalignanttumors,and tumor cell proliferation,anoikis, invasion andmetastasis of malignantbehavior. P19protein is atumorsuppressorgene,canspecificallyinhibitthekinaseactivityofG1CDK4/6,toprevent thecellsfromG1toSphasetransition,andcellcycleregulation,proliferation,apoptosis, migration,invasionandmetastasis,andangiogenesiscloselyrelated.DetectionofANXA1, P19genesexpressioninthegallbladder,gallbladderbenigntissue.Exploretherelationship betweentheoccurrenceofthedevelopmentofthegallbladderandgallbladdercancerclinical pathologicalparameters.Forfurther researchanddiscussionontheoriginanddevelopmentof gallbladderrelatedgenes,findingtheeffectivemethodofdiagnosisandtreatmenthascertain clinical value and theorysignificance.Methods1.CollectedfromJanuary2006toJuly2011,theGeneralHospitalof NingxiaMedicalHepatobiliarySurgerysurgicaltreatmentandclinical,pathologic,and follow-updataof56casesofcompletegallbladdercarcinoma,paraffin-embeddedspecimens, alsocollectedoverthesameperiodduetocholecystitisorchroniccholecystitisbenigndisease cholecystectomyspecimensof56cases.ByimmunohistochemistryS-Pmethodtodetectof ANXA1andP19proteinexpressionanalysisofexperimentalresultsusingSPSS11.5 statistical