apelin通过调节自噬促进脂肪间充质干细胞生存和功能的机制分析word格式论文.docx

3.2.2Apelin 减少心衰引起的心肌重构作用193.2.3Apelin 降低血压作用203.2.4Apelin 的抗缺血再灌注损伤作用203.3Apelin 的细胞保护作用203.4Apelin 对自噬的影响204 干细胞分子影像研究··········································································214.1 概述···························································································214.2 分子影像在干细胞研究中的应用·····················································224.2.1 干细胞的MRI成像（磁性纳米颗粒标记与示踪）224.2.2 PET和SPECT成像（放射性核素标记与示踪）244.2.3 干细胞的荧光成像···································································244.2.4 荧光分子断层成像(FMT) 244.2.5 自发荧光断层分子影像(BLT) 254.2.6 生物发光成像（bioluminescenceimaging，BLI）254.2.7 干细胞的多模态成像································································254.2.8 各种成像示踪技术的优缺点比较·················································264.2.9 对干细胞体内致瘤性及安全性的分子影像评价······························264.2.10 分子影像学在研究干细胞移植免疫排斥反应方面的应用前景··········27正文······························································································28第一部分Apelin 对脂肪间充质干细胞在后肢缺血小鼠体内生存和功能的作用···281 材料281.1 实验动物·················································································281.2 主要实验仪器···········································································281.3 主要实验试剂···········································································282 方法····························································································292.1 脂肪间充质干细胞分离培养与鉴定···············································292.2 建立后肢缺血动物模型·······························································292.3 实验分组·················································································292.4ADMSCs 细胞移植292.5 激光多普勒扫描检测后肢缺血模型血流··········································302.6 生物发光成像检测细胞存活·························································302.7 荧光素酶活性检测细胞活性·························································302.8 组织学观察细胞在组织内存活······················································302.9 免疫组织化学染色检测新生血管生成·············································302.10 自截肢率评价缺血程度·······