bacillus amyloliquefaciens m1菌株生产的抗菌脂肽对耐药性海洋病原弧菌抑菌活性的研究word格式论文.docxVIP

优秀毕业论文 精品参考文献资料 的稳定性较好，其最小抑菌浓度为12.5 μg/mL。 对M1菌株纯化后的抗菌脂肽的抑菌机理进行初步探究：纯化后的抗菌脂肽不仅 可以杀死完整的鳗弧菌细胞，还能杀死鳗弧菌细胞的原生质体，这说明抗菌脂肽可能 是通过破坏细胞膜来杀死细菌的。电镜检测发现，被抗菌脂肽作用过的鳗弧菌细胞表 面出现破损，细胞出现降解，细胞内容物释放，导致细胞死亡，说明抗菌脂肽能破坏 鳗弧菌细胞表面结构。荧光检测发现抗菌脂肽作用过的鳗弧菌细胞可以被染料PI染色， 进一步证明抗菌脂肽能破坏细胞膜结构，改变细胞通透性，破坏细胞的完整性，进而 杀死整个细胞。 关键词：解淀粉芽孢杆菌；脂肽； 耐药性； 病原弧菌；抑菌机理  Antibacterial activity of the lipopetides produced by Bacillus amyloliquefaciens M1 against multidrug-resistant Vibrio spp. isolated from diseased marine animals Abstract Bacillus amyloliquefaciens could produce a variety of lipopeptides, which have significant antibacterial, antifungal and antimycoplasma activity. The lipopeptide is composed of lipophilic hydroxyl-fatty acid chain and hydrophilic peptide chain. Antibacterial lipopeptide has great potential in the fields of biocontrol in agriculture, therapeutic, and environmental management. But it is still unknown if they have killing activity against the pathogenic Vibrio spp. isolated from diseased marine animals. Vibriosis could cause significant economic losses in mariculture. Different kinds of antibiotics, which would encourage the natural emergence of bacterial resistance, are used to prevent or cure the bacterial diseases in marine animals. So it is urgent need to find a natural and safe antibacterial substance that will kill the pathogenic Vibrio spp. with multidrug-resistant profiles. In this work, the M1 strain with the strong antibacterial activity was isolated from Mangrove and examined against 9 species of pathogenic Vibrio spp. isolated from diseased marine animals. By using conventional biochemical identification and molecular biology method, the M1 strain was identified to be one strain of B. amyloliquefaciens. It was found that all the Vibrio spp. showed resistance to several different antibiotics, suggesting that they were the multidrug resistance. In order to produce the maximal lipopeptides by the bacterial strain M1, a new and cheap medium which contained 1.5% wheat flour and 1