avastin抑制大鼠角膜新生血管的实验分析word格式论文.docxVIP

ResultsThe ratio of corneal epithelial defect area showed no significant difference (P0.05) between experimental group A and control group A at 12h,18h and 24h.Corneal clouding,edema,epithelial defect were not found after dropped with 5g·L-1Avastin eye drop on normal rat,s cornea.The corneal histological structure and ultramicrostructure were normal without the inflammatory cell infiltration in all corneas compared with control group B.The area of corneal NV was smaller in Avastin group，Avastin+dexamethasonegroup and 0.1% dexamethasone group in 3,6 and 12 days than 0.9% NaCl solution group(all P0.01).Infiltration of inflammatory cells and neovascular were detected in 0.9% NaCl solution group,but little of inflammatory cells and neovascular were found in Avastin group,dexamethasone group and Avastin+dexamethasone group.Expression of VEGF was much high in 0.9% NaCl group compared with dexamethasone group and Avastin group,and that in Avastin+dexamethasone group was weaker.No significant difference in corneal NV area was found between 0.1% dexamethasone group and Avastin group (P0.05).ConclusionsThere was no effect on the healing rate of rat corneal epithelial after short-term ocular application of 5g·L-1Avastin.The rat cornea structure didn,t change after short-term ocular application of5g·L-1Avastin.Topical application of Avastin,dexamethasone had inhibit corneal NV significantly.Avastin and dexamethasone show a synergistic effect in suppressing the expression of VEGF and inflammation cells.Avastin had the effect of anti-inflammatory.Key wordsAvastin;cornealepithelialdefect;healingrate;dexamethasone;corneal neovascularization中英文对照缩略词表NVneovascul ar i zat i on新生血管HEhemat oxyl i n and eosi n苏木素和伊红PBSphosphat e buff er sol ut i on磷酸盐缓冲液VEGFvascul ar endot hel i al gr owt h f act or血管内皮细胞生长因子VEGFRvascul ar endot hel i al gr owt h f act or血管内皮细胞生长因子r ecept or( KDR/f l k- 1)受体( KDR/f l k- 1)I Li nt er l euki n白细胞介素FGFbasi c f i br obl ast gr owt h f act o