dna纳米材料生物探针研制及其在dna和细胞检测中应用word格式论文.docxVIP

AuNPs structure.Chapter II, The new DNA biosensor is constructed based on the bridge-type Tri-AuNPs. As the novel bridge-type Tri-AuNPs structure，the Tri-AuNPs bio bar codes DNA probe was constructed. Taking advantage of amplification effects of one-to-one recognition Tri-AuNPs bio bar codes DNA probe and the one-to-one hybridization reaction between the probe DNA and the target DNA, we built one creative one-to-one recognition high-sensitivity DNA bio-sensors.This DNA biosensor could detect target DNA with the detection limit of 53 aM. These studies contribute to the combination of DNA sensors and DNA materials, appearrence of new high-sensitivity, high-selectivity DNA biosensors and DNA material as well as the application in other biological detection.Chapter III, the work proposes a strategy which aims to construct a DNA functional material network in the surface of cancer cell membrane. It is also consider that the network of DNA-functional materials which wrapped the cancer cell. The DNA-functional network mainly consists of two parts, first: multi-unit flexible polymeric DNA fragment, including the cells aptamer DNA which recognize the cancer cell ,the linker DNA which connect chains together to form a linear DNA polymerization unit. Second: DNA-CdTe QDS that DNA complex with CdTe QDS. This DNA-CdTe QDS can be combined into multi-unit flexible polymeric DNA to help the linear DNA polymerization unitconnected into a network, while the introduction of CdTe QDS made thesynthetic flexible polymeric DNA material fluorescence. Our synthesis of this DNA functional materials in the cancercell surface, make cancer cells be completely wrapped and clearlyobserved in the fluorescence microscope. Finally confirmed the initial feasibility of this package strategy by observation and through this material packages, we successfully achieved the purpose of analysing the process of cell life activities using fluorescence imaging.KEYWORDS: DNA; DNA nanotechnology; Tri-AuNPs bio