针刺肝俞对大鼠原位肝移植急性排斥的影响研究.docVIP

针刺肝俞对大鼠原位肝移植急性排斥的影响研究 丁利民1 邓丽珊2# 卢泳1 （1江西省人民医院 南昌330000；2江西省残疾文化体育管理中心 南昌330000） 摘要：目的：探讨针刺肝俞对大鼠原位肝移植急性排斥反应的影响。方法：建立肝移植急性排斥模型，80只模型大鼠数字分为对照组生理盐水组、治疗A组针刺肝俞组、治疗B组他克莫司组、治疗C组针刺肝俞+他克莫司组，每组20只。治疗A组针刺肝俞穴，1次/d，每次20 min；治疗B组给予他克莫司0.05 mg/g·d）灌胃，1次/；治疗C组给予他克莫司和针刺肝俞。观察各组大鼠生存率及生存时间，术后第7天取腔静脉血检测肝功能各项指标TBiL（总胆红素）、DBiL结合胆红素、AST谷草转氨酶、ALT谷丙转氨酶、ALP碱性磷酸酶及GGT谷氨酰转肽酶。结果与对照组相比，各组的术后存活时间明显延长，均明显下降P＜0.05；与治疗A组比较，治疗B和C组的术后存活时间明显延长，肝功能指标也显著下降P＜0.05；与治疗B组比较，C组的术后存活时间明显延长，其肝功能各项指标明显下降P＜0.05。结论针刺肝俞有效地减轻大鼠肝移植急性排斥反应，提高大鼠术后生存时间，其可应用于临床抗肝移植急性排斥反应治疗。 关键词大鼠肝移植；急性排斥反应；生存率；肝功能 The Study of Impact of Acupuncture Ganshu Point on Acute Rejection after Orthotopic Liver Transplantation in Rats DING Li-min1, DENG Li-shan2#, LU Yong1 (1The peoples hospital of Jiangxi province, Nanchang330000, 2The disabled culture sports management center in Jiangxi province, Nanchang330000) Abstract: Objective: To explore the impact of acupuncture Ganshu point on acute rejection (AR) after orthotopic liver transplantation (OLT) in rats. Methods: The 80 rats were divided into four groups by random number table: control group (normal saline group), acupuncture Ganshu point group (group A), tacrolimus group (group B), tacrolimus and acupuncture Ganshu group (group C), 20 in each group. The model of OLT with AR was established in 80 rats (Wistar donors and recipients). Given acupuncture Ganshu in rats of group A, one time a day, 20 minutes each time; given tacrolimus intragastric administration of 0.05mg/(kg·d) one time a day to rats of group B; given tacrolimus 0.05 mg/(kg·d) intragastric administration, one time a day and acupuncture Ganshu (one time a day, 20 minutes each time) in rats of group C. Analysis the survival rate of four groups, vena cava blood was taken to measure the levels of TBiL, DBiL, AST, ALT, ALP, GGT on postoperative day 7. Results: Compared with the control group, the survival time of rats treated with each group was significantly prolonged, and the indexes of liver function were significantly