eif5a2蛋白在人脑胶质瘤的表达和意义word格式论文.docx

Expression of EIF-5A2 protein in human brain gliomaAbstractBackgroundGliomaisthemostcommonprimaryintracranialtumor,whichtakesupabout 35.26%—60.96%inallkindsofbraintumors.Themajortherapymethedofqlivwais surgicaloperation.However,becauseoftheinfiltrativegrowthofthetumor,it’sdifficult toberesectedcompletely;thetumoriseasytorecuraftersurgery.Therefore,the pathogenesis,pathologyandcomprehensivetherapyofgliomahavealwaysbeingan investigativeissueforneurosurgeons.Inaddition,recurrenceandmetastasisofglioma,are hottopicsthatareneededtobesolved.Moreover,searchingfortheinvasiveness metastasisrelatedspecificgeneandprovidingearlydiagnosisandtherapyarethe keysfor decreaterecurrenceandcucveatesurvivalrate.Evidencesprovidedbyrecent cytogeneticalstudiesdemonstratedthattherearehigh-frequencyamplificationsof chromosome3qorpartly3qregioninmalignantepithelialtumorsderivingfromheadand neck,esophagus,breast,gastrointestineandbladder.Itindicates thatchromosome3qmay containsingleormultiplekeyoncogenesincertainDNAamplificationregion. Amplificationandirregularexpressionmayplayanimportantroleintumorgeneration.In 2001,Guanet.alclonedanewcandidateoncogeneEIF-5A2from3q26.2 overamplificationregioninovarian cancer cellviaemploying chromosome microdissectionandscreen-hybridizationtechnique.EIF-5A2wasnamedbecausethe proteinencodingbyithas82%homologywithEIF-5A2whichistheeukaryoninitiation factorPreviousinvestigationsindicatethatEIF-5Ageneplaysanimportantroleincell generationandproliferation,WhenEIF-5Aproteinwasinhibitedintheovarianepithelial cellofChinesehamster,thegenerationandproliferationoftheepithelialcellwerealso significantly inhibited, whichmay lead to cell apoptosis. Tumor promoting effect ofEIF-5A2has beenprovedinvivoandinvitro.EIF-5A2,amemberofeukaryoticinitiation factorfamily,presentsweaklyexpressioninnormaltestisandbraincell.Andhigh expressionincertainhumancancercelle.g.ovariancancerandcoloncancer.andthehigh expressionisrelatedtothetumors’clinicalstages.ItwasreportedthatEIF-5A2 overexpressi