epcam和ecad在胆囊癌中的表达及其与胆囊癌侵袭能力的关系word格式论文.docx

Ep-cam和E-cad在胆囊癌中的表达及其与胆囊癌侵袭能力的关系中文摘要目的：探讨上皮细胞粘附分子（Ep-cam）和上皮型钙粘附蛋白（E-cad）在胆囊癌中的表达及与其侵袭转移的关系。方法：1.胶原酶消化法原代培养胆囊癌细胞。 2.细胞侵袭实验（Transwell）检测胆囊癌细胞的侵袭能力。3.应用RT-PCR检测具有不同侵袭力胆囊癌细胞Ep-cam及E-cad基因表达。4.应用Western-blot检测具有不同侵袭力胆囊癌细胞Ep-cam及E-cad蛋白表达。结果：1、原代培养法可以培育出可传代的胆囊癌细胞。2、各原代培养出的胆囊癌细胞侵袭能力具有差异（P0.05)3、Ep-cam与E-cad的表达与胆囊癌细胞的侵袭能力有关，Ep-cam表达越高，胆囊癌细胞的侵袭力越高(P0.05)；E-cad表达越高，胆囊癌细胞的侵袭力越低(P0.05)。4、侵袭力无明显差异的胆囊癌细胞Ep-cam及E-cad的表达无明显差异(P0.05)结论：1、Ep-cam及E-cad的表达变化与胆囊癌的侵袭力有良好的相关性,表现为Ep-cam的表达与胆囊癌的侵袭能力呈正相关，E-cad的表达与胆囊癌的侵袭能力呈负相关。2、胆囊癌组织中Ep-cam蛋白表达与E-cad蛋白表达二者呈负相关。3、联合检测Ep-cam与E-cad在胆囊癌中的表达对评价胆囊癌的侵袭转移能力有一定价值。关键词：胆囊癌、原代培养、侵袭力、Ep-cam、E-cad、RT-PCR、Western-blot、TheexpressionofEp-camandE-cadinthegallbladdercarcinomaandtherelationshipwiththeinvasionabilityAbstractObjective:Toinvestigatetheexpressionoftheepithelialcelladhesionmolecule(Ep-cam)andtheepithelialtypecalciumadhesionproteins(E-cad)ingallbladdercarcinomawithinvasionability.Method:1.Gallbladdercancercellsinprimaryculture.Transwellexperimentsdetectgallbladdercarcinomacellinvasionability.RT-PCRdetectthe expressionof Ep-camandE-cadgeneingallbladdercarcinomacellswhichhavedifferentinvasionability.Western-blotdetecttheexpressionofEp-cam andE-cadproteiningallbladdercarcinomacellswhichhavedifferentinvasionability.Results:1.Primaryculturemethodcanbreedpassageofgallbladdercarcinomacells.2.Primarycultured gallbladder carcinomacells havedifferentinvasionability.(P0.05)TheexpressionofEp-camandE-cadareassociatedwiththeinvasionabilityofgallbladdercarcinomacells,thehighertheEp-camexpression,thehighertheinvasionability(P0.05);thehighertheE-cadexpression,thelowertheinvasionability(P 0.05).TheexpressionofEp-camandE-cadingallbladdercarcinomacells,whichhavetheSimilarinvasionability,werenotsignificantlydifferentnosignificantdifference.(P0.05)Conclusion:1.ThereisagoodcorrelationbetweentheexpressionofEp-cam\E-cadandtheinvasionabilityofgallbladdercarcinomacells;TheEp-camexpressionisassociatedwithgallbladdercarcinomainvasionwaspositively;TheE-cadexpressionisassociate