fasudil对eae小鼠脊髓内细胞rockⅱ表达影响word格式论文.docx

significantlydecreasedwhencomparedwiththoseofEAEgroup(2.24±0.26vs3.80±0.78,P0.05;15.55±1.50vs 13.35±1.309,P0.01).ThediseasedidnotappearinmiceadministeredbyFreundadjuvantandtheweightofthemiceincreased.HEstainingshowedmarkedinflammatorycellinfiltrationinspinalcordsofEAEmice.Thescoreof inflammatorycellinfiltrationaccordingtoOkudastandardinFasudiltreatedgroupwasstatisticallysignificantlyincreasedwhencomparedwiththoseofEAEgroup(20.22±1.56vs31.78±1.04,P0.05）.Luxol FastBluestainingshowedobviousdemyelinationinEAEgroupthantheEAEgroup. Thedegreeofdemyelination waspositivelycorrelatedwiththeseverityofthedisease.IndirectimmunofluorescenceshowedthatRock-Ⅱexpressedinspinalcords ofmiceineachgroup.ThehighertheclinicalscoreinEAEmice,themoretheexpressionofRock-Ⅱ.ItshowedthattheexpressionofRock-Ⅱis obviouslyinvascularendothelialcells.ThenumberofdotpositioningofRock-ⅡinFasudiltreatedgroupwasstatisticallysignificantlydecreasedwhencomparedwiththoseofEAEgroup(20.22±1.56vs31.78±1.04,P0.01).Conclusion:MOG35-55caninduceEAE successfullyinC57BL/6mice.Rho/Rocksignalingpathwayinvolved inthepathogenesisofEAE.Fasudilcouldsignificantlydecreasetheincidencerate,relievetheclinicalseverityofEAEandlessen inflammatorycellinfiltrationanddemyelination.Fasudil could significantly decrease the expression of Rock-Ⅱwhich may be one ofmechanismthat FasudilcantreatEAE.Key word: experimental autoimmune encephalomyelitis, multiple sclorosis, Rho kinase,Rock-Ⅱ,Rho/Rocksignalingpathway,Fasudil英文缩略词表(Abbreviation)缩略词BBB英文全称blood-brain-barrier中文名称血脑屏障BSACDCFACKCNSEAEECERMFITCGTPIgGMOGMLCPMLCbovineserumalbuminclusterofdifferentiationCompleteFreund’sadjuvantcytokinecentralnervoussystemexperimentalautoimmuneencephalomyelitisendothelialcellsezrin- radixin-moasinfluoresceinisothiocyanateguanosinetriphosphateimmunoglobulinGmyelinoligodendrocyteglycoproteinpeptidemyosinlight chainphosphatasemyosinlightchain牛血清白蛋白分化群完全弗氏佐剂细胞因子中枢神经系统实验性自身免疫性脑脊髓炎内皮细胞细胞骨架连接蛋白异硫氰酸荧光素三磷酸鸟苷免疫球蛋白G髓鞘少突胶质细胞糖蛋白肌球蛋白轻链磷酸酶肌球蛋白轻链MSmultiplesclerosis多发性硬化PBS