鬼针草煎剂对h 22肝癌荷瘤小鼠抑瘤率及免疫功能影响的实验研究word格式论文.docxVIP

Sher b decoct i on Bi dens i nhi bi t or y r at e of H22 pr i mar yhepat i c i n mi ce And i mmune f unct i on i n exper i ment alst udyAbst r actObj ect i ve: Obser vat i on of her b Bi dens decoct i on H22 t umor- bear i ng mi ce ani mal model s of pr i mar y hepat i c cancer i nhi bi t i on r at e and i mmune f unct i on, and t o expl or e Bi dens mechani sm of ant i - pr i mar y hepat i c cancer , i n or der t o f ur t her expl or e t he Bi dens l ay t he f oundat i on f or pr event i on and t r eat ment of pr i mar y hepat i c cancer .Met hod: Sel ect heal t hy Kunmi ng mi ce wer e 60 mal e and f emal e, wer e r andoml y di vi ded i nt o 6 gr oups of 10 each, mal e and f emal e i n hal f , r espect i vel y, t he bl ank gr oup, model gr oup, cycl ophosphami de gr oup, Bi dens hi gh dose gr oup, Bi dens mi ddl e dose gr oup , Bi dens l ow dose gr oup. Col l ect ed under st er i l e condi t i ons i nocul at ed mi ce 7d of H22 asci t es asci t es by cent r i f ugat i on mi l ky whi t e. Tr ypan bl ue st ai n sol ut i on, l i vi ng cel l count, cel l vi abi l i t y 95%. Except f or t he bl ank i n t he ot her gr oups, i n st er il e condi t i ons i n t he r i ght axi l l ar y subcut aneous i nj ect i on of 0. 2ml per mouse t umor cel l suspensi on, made of sol i d t umor model . Al l mi ce wer e gi ven nor mal di et, except t he bl ank gr oup, cycl ophosphami de gr oup r ecei ve i n t r aper i t oneal i nj ect i n of 0. 025g/ Kg/ d; Bi dens hi gh, medi um and l ow dose gr oup wer e gi ven 46. 4g/ kg/ d, 13. 92/ kg/ d, 4. 64/ kg/ d Bi dens decoct i on; model gr oup wer e gi ven equal vol ume of nor mal sal i ne. Cont i nuous admi ni st r at i on of 14d, st oppi ng t he next day, t he eye measur i ngbl ood i nt er l euki n - 2 ( I L- 2) , t umor necr osi s f act or α ( TNF- α) ; peel i ng t het umor , t umor i nhi bi t i on r at e; pr of i l e cont r ol spl een and t hymus, cal cul at ed spl een i ndex and t hymus i ndex.Res