分子基因选殖实习手册.docVIP

PAGE PAGE 28 『分子基因選殖』實習手冊 （一）Mini-M plasmid DNA extraction system (VIOGENE) Grow 1 to 5ml plasmid-containing bacterial cells in LB medium with appropriate antibiotics overnight (12-16 hours) with vigorous agitation ↓ Pellet the cells by centrifuging for 1-2 minutes. Decant the supernatant and remove all medium residue by pipet. ↓ Add 250 ?l of MX1 Buffer to the pellet, and resuspend the cells completely by vortexing or pipetting. ↓ Add 250 ?l of MX2 Buffer and gently mix (Invert the tube 4 ~ 6 times) to lyse the cells until the lysate becomes Clear. Incubate at room temperature for 1-5 minu