临床病理沟通.ppt

* HL: the role of microenvironment HRS T B B Cell component (CD 20 expression) 20% -70% * Rituximab Treatment of Hodgkin Disease MDACC Stanford Germany The Target Normal B cells Tumor cells Tumor cells Eligibility Relapsed New or Relapsed Relapsed LP NS or MC LP or CD20+ NS/ MC Dose/Schedule 375 mg/m2 375 mg/m2 375 mg/m2 Weekly x 6 Weekly x 4 Weekly x 4 Evaluable (N) 22 22 14 (10 LP) CR 1 10 8 PR 4 12 4 Overal RR 23% 100% 86% 谢 谢 Subcutaneous panniculitis-like T-cell lymphoma WHO分类原则 细胞谱系是疾病定义的出发点 B、T或 NK 细胞 每种疾病都是具有临床和实验室特征的独立实体 形态学 免疫表型 遗传学特征 临床表现与病程 疾病累及部位常常是重要的生物学特征性标志 临床特征的应用是一个新的方面；诊断并非凭空而造 * 50 * This slide shows the findings of various diagnostic methods. The initial evaluation of a patient with possible NHL should include a complete history and physical examination, blood counts and serum chemistries, imaging studies, and a bone marrow aspirate and biopsy. Diagnostic methods performed on the biopsy specimens include a routine histologic study, immunoperoxidase staining, flow cytometry, cytogenetics, and molecular biologic methods (Southern blotting, polymerase chain reaction [PCR]). Fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH) are newer techniques that are useful for identifying chromosomal abnormalities, including translocations. FISH is now widely used to identify aberrant DNA sequences in both intact cells, either in interphase or metaphase, and isolated chromosomes. The detection of chromosomal aberrations can be performed at the single-cell level among both dividing and nondividing cells, therefore eliminating the need of metaphase preparations. Compared with FISH, CGH has much greater multiplexing capabilities. This technology examines numerous regions of the genome simultaneously for changes in DNA copy number, identifying complex patterns of gains and losses within the genome. CGH quantifies relative DNA copy differences between hybridized test DNA labe