HPLC同时测定妇炎康复片3种主要抗炎成分含量.docVIP

HPLC同时测定妇炎康复片3种主要抗炎成分含量 作者：汪选斌1,湛晓明2,王明仲3 单位：1郧阳医学院附属人民医院中药药理实验室，湖北 十堰 442000;2湖北中医学院，湖北 武汉 430000;3襄樊市中心医院，湖北 襄樊 441000) 【摘要】 目的:建立HPLC同时测定妇炎康复片中芍药苷、橙皮苷及黄芩苷含量的方法。方法:采用HPLC条件为：色谱柱为Phenomenex C18柱(250 mmtimes;4.60 mm，5mu;m);流动相：乙腈-磷酸二氢钾水溶液(0.05 mol/L，磷酸调pH至3.0)(梯度洗脱:0 min,17∶83;10 min,17∶83;15 min,22∶78;30 min,30∶70);流速：1.0 mL/min;柱温：40 ℃;检测波长：231 nm。结果:芍药苷的线性范围为0.0533～0.4800 mu;g (r=0.9997)，橙皮苷的线性范围为0.0798～0.7182 mu;g(r=0.9999)，黄芩苷的线性范围为0.3600～3.420 mu;g(r=0.9999);平均加样回收率，芍药苷为99.49%，RSD=1.70%(n=5)，橙皮苷为99.59%，RSD=0.41%(n=5), 黄芩苷为99.92%，RSD=1.26%(n=5)。结果测得3厂家妇炎康复片中每片含芍药苷为1.0656，1.4455，0.7042 mg;橙皮苷为0.5383，0.7676，0.4403 mg;黄芩苷为3.9544，7.0312，2.8877 mg。结论:该方法简便、快速、准确，可用于妇炎康复片的质量控制。 【关键词】 妇炎康复片;芍药苷;橙皮苷;黄芩苷;高效液相色谱法 　Simultaneous Determination of Three Main Antiinflammatory Constituents in Fuyankangfu Tablets by HPLC WANG Xuanbin1，ZHAN Xiaoming2，WANG Mingzhong3，XIAO Yuqing1，LI Hongliang1，WANG Shanshan1，LIU Ming1 (1Laboratory of Chinese Herbal Pharmacology,Renmin Hospital,Yunyang Medical College, Shiyan, Hubei 442000;2Traditional Chinese Medicine College of Hubei,Wuhan 430000;3The Central Hospital of Xiangfan,Xiangfan,Hubei 441000,China) 　　Abstract:Objective To establish a HPLC method for determining the contents of paeoniflorin, hesperidin and baicalin in Fuyankangfu Tablets. Methods HPLC was performed on a PhenomenexC18 column(250 mmtimes;4.60 mm，5 mu;m),and the mobile phase was composed of acetonitrile0.05mol/L KH2PO4 (adjusted to pH 3.00 with H3PO4)with gradient elution(0 min,17∶83;10 min,17∶83;15 min,22∶78;30 min,30∶70).The flowrate was 1.0 mL/min. The operating temperature was maintained at 40 ℃ and the detection wavelength was 231 nm. Results The linear ranges of paeoniflorin,hesperidin and baicalin were 0.0533～0.4800 mu;g(r=0.9997), 0.0798～0.7182 mu;g(r=0.9999) and 0.3600～3.4200 mu;g (r=0.9999),respectively.The average recovery rate of paeoniflorin was 99.49%(RSD=1.70%，n=5), hesperidin was 99.59%(RSD=0.41%，n=5)and baicalin was 99.92%(RSD=1.26%，n=5).The con