SYKmRNA在卵巢癌组织中的表达及其临床意义.docVIP

SYKmRNA在卵巢癌组织中的表达及其临床意义 作者：仵妍,马德花,赵淑萍 单位：青岛大学医学院附属医院妇科， 山东 青岛 266003 【摘要】 目的：探讨SYKmRNA在卵巢癌组织中的表达及其与临床病参数之间的关系。方法: 采用逆转录聚合酶链反应(RT-PCR)测定40例卵巢癌组织、20例卵巢良性上皮性肿瘤组织及20例正常卵巢组织中SYKmRNA的表达。结果: 卵巢癌组织中SYKmRNA的表达水平明显低于正常卵巢组织及卵巢良性上皮性肿瘤组织，差异有显著性意义(Plt;0.05);SYKmRNA的表达水平在卵巢良性肿瘤及正常卵巢组织之间无明显差异(t=0.21,Pgt;0.05)SYKmRNA阳性表达率与卵巢癌的临床分期、组织分级、淋巴转移均高度相关(Plt;0.01)。浆液性卵巢癌中SYKmRNA阳性表达率明显低于其在粘液性卵巢癌中的表达率(X2=4.57，Plt;0.05)。结论: SYKmRNA表达缺失或减少可能与卵巢癌的发生、浸润、转移及恶性程度密切相关，可能是一潜在肿瘤抑制因子。 【关键词】 卵巢肿瘤; SYK; 肿瘤抑制因子 　　The Relation Between the Expression of SYKmRNA in Ovarian 　　Carcinoma Tissues and the Clinical Significance 　　WU Yan, MA De-hua, ZHAO Shu-ping 　　(The Affiliated Hospital of Medical College of Qingdao University , 　　Shandong Qingdao 266003, China) 　　Abstract： Objective:To study the expression of SYKmRNA in ovarian carcinoma tissues and its relation with the clinicopathological features. Method: The expression of SYK gene in ovarian carcinoma tissue (n=40), benign epithelial neoplasms(n=20) and normal ovarian tissue(n=20) were detected by RT-PCR technique. Result: The level of the SYKmRNA was obviously lower than that in the benign ovarian tumor and the normal ovarian tissue(Plt;0.05);but the difference of the SYKmRNA levels between the benign ovarian tumor and the normal ovarian tissue had no significance(t=0.21,Pgt;0.05). The positive rate of SYKmRNA in ovarian carcinoma was significant associated with the clinical stage. histologic grade and lymph node metastasis (Plt;0.01);The positive rate of SYKmRNA was lower in ovarian serous carcinoma than that in ovarian mucinous carcinoma(X2=4.57，Plt;0.05).Conclusion: The miss of SYKmRNA the reduced of SYKmRNA may be associated with the occurrence, invasion, metastasis and the malignant of ovarian carcinoma, SYK may be a potential tumor suppressor in ovarian carcinoma. 　　Key words： Ovarian neoplasms; SYK; Tumor suppressor 　　卵巢癌是一种严重威胁妇女健康的恶性肿瘤，绝大多数卵巢癌患者早期症状不明显，约有70%的患者就诊时已属晚期，具有恶性程度高、病情发展快、预后差的特点，死亡率居女性生殖系统恶性肿瘤首位，5年生存率仅25～30%。因此，研究卵巢癌的发病机制，阻断其发生过程的关键环节，寻