大鼠孕期铁缺乏模型建立及对仔鼠发育影响.docVIP

大鼠孕期铁缺乏模型建立及对仔鼠发育影响 【摘要】目的 构建大鼠孕期营养性铁缺乏模型，探讨母鼠缺铁贫血对仔鼠生长发育的影响。方法 将6周龄健康Wistar雌性大鼠随机分为2组,实验组即低铁组喂饲低铁饲料,对照组喂饲富铁饲料。4周后与健康成年Wistar雄鼠同笼交配，同笼前检测体重、血红蛋白(Hb)、红细胞分布宽度(RDW-CV)、铁蛋白等值(Fn),待雌鼠产仔观测雌鼠受孕率、雌鼠产仔活产率、活胎数及幼仔断乳日存活率及其生长状况。结果 干预4周后(同笼前)低铁组血红蛋白(Hb)为(123.07±12.18)g/L，体重等值均低于对照组(P0.05);红细胞分布宽度(RDW)为(26.53±5.31)%，游离红细胞原卟啉(FEP)为1.97±0.41，均高于对照组(P0.05)。对照组雌鼠与低铁组雌鼠受孕率相同，均为83.3%;对照组仔鼠与低铁组仔鼠相比，活胎率和存活率及其生长速度差异均有统计学意义(P0.05)。结论 母鼠缺铁性贫血可影响仔鼠生长发育。 【关键词】 孕期;铁缺乏;大鼠模型;仔鼠发育Effect of iron deficiency duing pregnancy on fetus development in rat LIU Min，MA Yajing,CHENG Jiang,et al.Key Laboratory of Xinjiang Endemic and Ethnic Diseases,Ministry of Education(Shihezi 832002,China) Abstract： Objective To establish a nutritional iron deficiency model in pregnancy rats and to study the effect of iron deficiency anemia on fetus development.Methods Six-month healthy female Wistar rats were randomly divided into two groups.The rats in experimental group and control group were fed with low iron or normal diet,respectively.After four weeks,the rats were mated with healthy adult male rats.Before the mating,levels of hemoglobin,erythrocyte hemoglobin distribution width,ferritin were detected.After the offspring were born,the conception rate,the development and growth of the newborn until weaning were observed.Results Hemoglobin and weight were lower in experimental group than those in control group (P0.05) before the mating.Erythrocyte hemoglobin distribution width and free erythrocyte protoporphyrin were higher in experimental group.The conception rates of two groups were the same(83.3%).There were significant differences in offspring survival rate,birth rate,and growth rate between the two groups(P0.05).Conclusion The nutritional iron deficient model in pregnancy rats was established successfully.Pregnant rats iron deficiency anemia can affect their fetus growth. Key words： pregnancy;iron deficiency;model for rats;rat fetus development 铁对维持机体正常的生理功能有重要意义〔1〕,但铁缺乏症却是一种全球范围内的常见病,多见于婴儿、儿童、妇女及贫穷的人群〔2〕。研究表明,缺