小鼠子宫内膜的组织培养.docVIP

小鼠子宫内膜的组织培养 【关键词】 子宫内膜 In vitro culture of mouse endometrium TAN DongMei1, TAN Yi1, ZHAO Jie2, AI Ling2, WANG ZhiBiao2 1Laboratory Animal Center, 2Ultrasound Engineering Institute of Medical Science, Chongqing University of Medical Science, Chongqing 400016, China 【Abstract】 AIM: To develop an in vitro culture model of mouse endometrium. METHODS: Mouse endometrium squeezed from the uterus horns were cultured at the gasliquid interface between F12/DMEM (1∶1) including 200 mL/L FBS, 17.5 nmol/L insulin, 63.5 nmol/L progesterone, 7.14 nmol/L estradiol, and 50 mL/L CO2 plus 950 mL/L air. The effec