基于ag@c核壳材料的生物电化学传感器分析-analysis of bioelectrical chemistry sensor based on ag @ c core-shell material.docxVIP
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基于ag@c核壳材料的生物电化学传感器分析-analysis of bioelectrical chemistry sensor based on ag @ c core-shell material
StudyonAg@Ccore–shellnanocompositebiosensorsAbstractAg@C/HRP/ITObiosensorwithoutspecialfilm-formingagenthasgoodresponseofH2O2.Thebiosensorhasagoodresponsesensitivityandlowdetectionlimit,thelinearrangeis1.0×10-7to1.0×10-4M(R=0.998),detectionlimitis0.2μM,responsetimeislessthan3s,whileKmequalsto37.5μM.WecanknowfromtheexperimentalresultsthatAg@C/HRP/ITObiosensorsspeedupelectrontransferbetweenHRPandtheelectrodedirectly,whichrevealAg@Cnanoparticleshavegoodbiocompatibleandbroadenapplicationprospectsinthefieldofenzymeimmobilization.Inaddition,thisbiosensorshowedawidedetectionrange,lowdetectionlimit,goodstabilityandanti-jamminginthedetectionofH2O2.Insummary,thisnewbiosensorcanbeeasilyconstructed,providedanewmethodforthedetectionofH2O2,andAg@Cnano-materialsarealsoexpectedtobeusedfortheimmobilizationofotherenzyme.Keywords:Ag@Cnanomaterialswithcore-shellstructure;tryptophan;horseradishperoxidase;biosensorsWrittenBy:Shu-XianMaoSupervisedBy:AssociateProfessorYu-MeiLongProfessorAn-PingDengIII目录第一章绪论................................................................................................................11.1生物传感器.....................................................................................................11.1.1生物传感器的发展...............................................................................11.1.2生物传感器的工作原理和器件............................................................21.1.3生物传感器的分类...............................................................................21.2电化学酶传感器.............................................................................................31.2.1电化学酶传感器的工作原理................................................................41.2.2电化学酶传感器的分类........................................................................41.2.3酶的固定化方法...................................................................................61.2.4电化学酶传感器的发展趋势和特点.....................................................71.3核壳材料..............................................................
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