荧光定量PCR技术与应用.pdf

Real-Time PCR -DNA -RNA Protein Genomic Sequence SNPs CNVs Methylation 2 • Genotyping Solution • PCR − microRNA − CNV − − TaqMan 3 Single Nucleotide Polymorphisms (SNPs) A Single Nucleotide Polymorphism (SNP) is a change in the nucleotide present at a single base in a known DNA sequence. ATGCATGCATGCATGCATGC G ATGCATGCATACATGCATGC A This G/A is a SNP 4 GT Market Positioning Markers • 500,000 • SNP • 100,000 • Candidate Region / Gene • Fine mapping • Disease association 10,000 • ADME / Tox • Trait selection and breeding, QC 1,000 • Large sample • ADME / Tox candidate gene / • Candidate Gene marker screening Commercialization/ 100 Commercialization/ Candidate Gene / Candidate Gene /