红树林耐酸真菌penicillium camemberti oucmdz-1492抗h1n1活性产物研究-study on anti - h1n1 active products of acid-tolerant mangrove fungus penicillium camembert i oucmdz - 1492.docx

17 个化合物的结构，其中 8 个新化合物。吲哚二萜类化合物 11 个（1-11）、5 个α-吡喃酮类（12-16）和 1 个酰胺类化合物。 运用体外及体内生物活性筛选模型，采用 MTT 法、SRB 法等方法评价所获得的单体化合物的抗肿瘤活性和抗流感病毒 H1N1 活性；采用琼脂稀释法评价化 合物对致病菌的抑菌活性。结果表明：化合物 1-3 和 5-10 对 H1N1 病毒有显著 的抑制活性，IC50 分别为 28.3，38.9，32.2，73.3，34.1，26.2，6.6，77.9 和 17.7 μM，其活性超过阳性对照利巴韦林。综上，本文对一株耐酸真菌的次生代谢产物进行了系统研究，共分离鉴定了 17 个化合物，包括 6 个新的吲哚二萜类化合物，1 个新的 α-吡喃酮类化合物和 1 个新的酰胺类化合物, 其中化合物 1-3、5-10 表现出显著的抗 H1N1 流感活性。 这些研究发现低 pH 条件下更利于菌株 OUCMDZ-1492 产生丰富的次生代谢产物, 增加其化学多样性, 进一步证实了菌株次生代谢产物可由体外 pH 调节，低 pH 可以激活某些沉默基因，促进抗 H1N1 病毒活性物质产生。关键词：耐酸天才真菌，次生代谢产物，抗 H1N1 活性Anti-H1N1 Secondary Metabolites from the Mangrove-Derived Aciduric Fungus Penicillium camembertiOUCMDZ-1492AbstractAciduric microorganism is an extremely important class of special habitat microorganisms. It is possible to produce a variety of unique bioactive substances and has potential value because it has different genetic backgrounds and metabolic pathways compared with ordinary microorganisms. The mangrove is a junction of land and sea typical ecosystem, its soil is acidic with rich microbial resources. The microorganisms of aciduric fungus from the mangrove soil have very different genetic and physiological characteristics compared with microorganisms in the other environment, they can produce unique secondary metabolites and has great potential for medicine. It is rarely reported about Studies on the microorganisms from mangrove acidic soil, the research is enticing.In order to discover bioactive new compounds derived from aciduric fungus, a study is carried out by integrated chemical screening and bioactive screening. The studies contained isolation of microorganisms from mangrove soil, screening talented strains from aciduric fungus, the fermentation conditions fumble of the target strains, the fermentation and compound separation, the structural elucidation and biosynthetic pathway speculation, bioactive evaluation and structure activity relationship evaluation of pure compounds.205 strains of aciduric fungi have been isolated from