左心辅助对急性左心衰犬心肌局部angii受体mrna表达的实验研究.pdfVIP

Abstract Objective :To examine the alteration of local AngII receptor(AT1/AT2) mRNA expression in left ventricular myocytes of acute left heart failure canine and left ventricular assist device(LVAD) treatment and its mechanism. Methods: 15 canines were randomly divided into 3 groups(5,5,5): Control HF HF+LVAD groups. Acute left heart failure model was induced by occluding the left anterior descending coronary artery (LAD) or LAD+left circumflex coronary artery(LCX). The LVAD group was treated for 3 hours by pneumatic LVAD, whose output was 70ml/kg/min.At the same time, the systematic mean aortic pressure (mAOP) and the ±dp/dtmax of the left ventricle were observed. 100mg myocardium was taken for the experiments from the left ventricle of each dog at 1, 2, 3h respectively. The expression of the local left ventricle AngII receptor (AT1/AT2) mRNA was tested by reverse transcription-polymerase chain reaction (RT-PCR). Results:Compared with the control group, the expression of the AT1 mRNA in ischemic left ventricle was decreased significantly in HF group after heart failure; AT2 mRNA in ischemic left ventricle was increased significantly in HF group after heart failure. At 1, 2, 3 hours after LVAD, the AT1 mRNA level of the HF+LVAD group was higher than that of the HF group’s, the AT2mRNA level of the LVAD group was lower than that of the HF group’s. After heart failure, the systematic mAOP and dp/dtmax of the left ventricle of the experimental dogs decreased markedly. In HF group, the systematic mAOP and dp/dtmax of the left ventricle was decreased gradually. In contrast, the systematic mAOP and dp/dtmax of the left ventricle was significantly increased in LVAD group treated by LVAD, but the level of 3rd-hour assi