hl60细胞凋亡的衍射图像量化参数初步研究-preliminary study on quantitative parameters of diffraction images of hl60 cell apoptosis.docx
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hl60细胞凋亡的衍射图像量化参数初步研究-preliminary study on quantitative parameters of diffraction images of hl60 cell apoptosis
Key words: Cell Apoptosis, Morphological Change, Diffraction-Image Flow Cytometry, Diffraction Image, Gray-Level Co-occurrence Matrix目录第一章绪论........................................................................................................11.1 细胞凋亡概述.........................................................................................11.2 细胞凋亡途径.........................................................................................21.2.1 死亡受体途径..................................................................................21.2.2 线粒体途径......................................................................................31.2.3 内质网途径......................................................................................31.3 细胞凋亡的相关因素.............................................................................41.4 细胞凋亡的检测方法.............................................................................51.4.1 形态学检测......................................................................................51.4.2 免疫组化检测..................................................................................71.4.3 生化检测..........................................................................................81.5 细胞凋亡与肿瘤...................................................................................101.6 本文的研究内容及目的.......................................................................10第二章HL-60 细胞凋亡的流式检测及荧光图像分析122.1 自由基与细胞凋亡...............................................................................122.2 实验部分...............................................................................................132.2.1 实验材料与仪器............................................................................132.2.2 HL-60 细胞培养及凋亡诱导 132.2.3 HL-60 细胞凋亡的流式检测 142.2.4 HL-60 细胞凋亡的荧光图像分析 142.3 实验结果与分析...................................................................................152.3.1 HL-60 细胞流式检测结果分析 152.3.2 HL-60 细胞凋亡的荧光图像结果分析 182.4 本章小结..................................
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