hoxa10及其辅因子pbx1 pbx2在子宫内膜良 恶性病变中的表达-expression of hox a10 and its cofactor pbx1 pbx 2 in benign and malignant endometrial lesions.docx

独创性声明本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到本人将承担本声明引起的一切法律后果。学位论文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。本论文属于保密□，在年解密后适用本授权书。不保密□。（请在以上方框内打“√”）学位论文作者签名：指导教师签名：日期：年月日日期：年月日目录一、中文摘要······································································1二、英文摘要······································································3三、正文············································································5前言············································································5材料与方法···································································8结果··········································································11附图··········································································14讨论··········································································24参考文献····································································29四、综述··········································································31参考文献····································································47五、致谢··········································································52HOXA10及其辅因子PBX1、PBX2在子宫内膜良、恶性病变中的表达华中科技大学同济医学院附属同济医院妇产科研究生张小娟导师钟刚教授摘要目的：1．检测HOXA10在正常增生期、简单增生期、复杂增生期、非典型增生期及子宫内膜样腺癌各期中的表达情况，探讨HOXA10表达水平在雌激素依赖型子宫内膜腺癌（即内膜样腺癌）发生发展中的意义。2.检测Pbx1和Pbx2在正常增生期子宫内膜、简单增生期、复杂增生期、非典型增生期及子宫内膜样腺癌各期的表达情况。3.分别比较Pbx1和Pbx2在各期子宫内膜病变中的表达差异，初步探讨二者与HOXA10在子宫内膜样腺癌发病中的可能作用。4．比较在雌、孕激素受体阳性和阴性的子宫内膜样腺癌中HOXA10表达水平，分析HOXA10和雌、孕激素受体的相关性。方法：随机选取符合条件的华中科技大学附属同济医院2007-2010年病理科病理结果确诊为正常增生期子宫内膜和各期病变的子宫内膜蜡块组织共118例，用免疫组化的方法检测其HOXA10、Pbx1和Pbx2的表达及分布情况，并通过Image-proplus图像分析系统对染色组织进行半定量分析。结果：1.免疫组织化学结果显示在正常的子宫内膜组织及病变的各期子宫内膜组织中腺上皮和间质细胞中HOXA10均有表达。2．从正常增生期到腺癌其表达总体上呈下降趋势，简单增生期和复杂增生期子宫内膜组织相比，HOXA10表达差异无统计学意义（P＞0.05），在非典型增生和子宫内膜样腺癌中表达下降更为明显（P＜0.05）。3．Pbx1和Pbx2在子宫内膜各期病变的腺上皮细胞核和间质细胞核中均有表达。4．Pbx1和