hpdgfahbdlt2gt双基因修饰bmscs及其对难愈性创面的促愈作用研究-study on hpdgfahdb2t 2 gt double gene modified bms cs and its healing promoting effect on refractory wounds.docx
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hpdgfahbdlt2gt双基因修饰bmscs及其对难愈性创面的促愈作用研究-study on hpdgfahdb2t 2 gt double gene modified bms cs and its healing promoting effect on refractory wounds
英文缩写一览表英文缩写英文金称中文名称AdvAdenovirusvector腺病毒载体BMSCsbonemarrow-deriv创mesenchymalstem骨髓间充质干细胞cellsBpBasepair碱黎对BSABov切.esenunalburnin牛Jfn.清蛋白cDNAComplem侃侃ryDNA1?.1DNACPECytopa伽ice舵ct细胞病现效应CMConditionedmedium条件培养基CVL.Crudevirusliquid粗制病毒液DAB33-diaminobeniz?ne二二氨基联苯黯DMEMlF12Dulbecωsmodifiedeag1esmedium/F12DMEMlF12培养液DMSODimethylsulfoxide工甲基亚枫DNADeoxyribonucleicacid脱氧核糖核酸EBEthidiumbromide澳化乙镀EGFPEnhanc创greenfluorescentprotein增强型绿色荧光蛋白GyGray戈瑞hBD2humanbeta-defens?n-2人F防御萦2HEHematoxylin-ωs?n与苏木精…伊红hPDGF-Ahumanplatelet-derivedgrowthfactorA人血小板源性生长因子AIRESIntemalribosomeen町site内部核糖体插入位点KDaK.ilo也ltons干道尔顿LBMTILuriaB笛tani3-(4,S-dime伽lyl刷azol-2-yl)-2.LB培养基四瞠氮蓝5.diphenyltetrazoliumbromideODPBSOpticaldensityPhosphatebalancedsolution光密度假磷酸鼓缓J中液PCRPolym衍asechainreaction聚合酶链反应PFURNAPl叫ue-formtingunitRibonucleicacid空斑形成单位核糖核酸Rl二PCRReversetranscriptionPCR逆转录聚合酶链反应SDSPolyacrylamidegelelectrophoresisSDS…聚丙烯瞰胶凝胶电泳TEMEDSodiumdodecylsulfateN,N,N,N巳tetramethylethylenediamineN,N,N,N…囚甲慕乙二旅TRlTCTe阳methylrhodamineiso伽i侃yanate四甲篡异碗氟酸罗丹明2第三军医大学研究生学位论文独创性声明秉承学校严谨的校风和科研作风,本人申明所呈交的论文是我本人在导师指导下进行的研究工作及取得的研究成果.据我所知,除了文中特别加以标班和致谢的地方外,论文中不包含其他人已经发表或撰写过的研究成果,也不包含为获得我校或其他教宵机构的学位或证书而使用过的材料,与我问工作的阿志对本研究所做的任何贡献均已在论文中作了明确的说明并表示谢意.申请学位论文与资料若有不实之处,本人承担一切相关责任.论文作者签名:却品一日期:rlι第三军医大学研究生学位论文版权使用授权书本人究会了解第三军医大学有关保护知识产权的规定,即:研究生在攻读学位期间论文工作的知识产权单位属第二军医大学.本人保证毕业离校后,发表论文或使用论文工作成果时署名单位为第二军医大学.学校有权保留并向回家有关部门或机构送交论文的复印件和磁盘,允许论文被查阅和借阅.学校可以公布学位论文的全部或部分内容(保密内容除外),可以采用影印、缩印或其他手段保存论文.论文作者籍华均在指导教附:每缉队日期:品4纠第三军阪大学硕士学位论文Theestab1ishmentofhPDGF-AIhBD2gene回modifiedBMSCsandtheapplica咀onofthecellstodifficultwoundhealingasacountermeasureAbstractTrea恤entforchronícwoundhealíngcomplicatedbypathologicalconditíons,suchasdíabetes,radíotherapyorlargeareadefectofskinand80on,hasbeenaωughchallengeínclínic.Trar呻lantationofskin,usuallyusedforthe阳atmentofwoundoflargearea,ísrestraínedbysomedisadvantagesínpractice.Normalwoundhealíngisdeterioratedbymultiplefactors,íncludíngdecreas创inflammatorycellinfiltration,reducedsecretíonofgrowthf如协rsandcollagensyn出e
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