hplc实用型简易激光诱导荧光检测器的研制及hplc审审uvd法测定乙二醇中甲醛和乙醛含量-development of practical simple laser-induced fluorescence detector by hplc and determination of formaldehyde and acetaldehyde in ethylene glycol by hplc and uvd method.docxVIP

摘要本学位论文共包括三章。第1章：绪论。主要介绍了荧光检测原理，激光诱导荧光检测器（LIFD）的组成、结构及与其它检测技术的联用，并对LIFD的发展做了展望。第2章：实用型简易激光诱导荧光检测器的研制及性能测试。基于正交型结构，研制了一种简易实用的高效液相色谱激光诱导荧光检测器。以930荧光光度计为骨架，借用岛津RF-535荧光检测池，配以自制可调稳压电源，分别以波长532nm和405nm的激光器为激发光源，以罗丹明B和荧光素为荧光试剂评价体系的性能。测试结果表明该检测器达到了设计要求，具有足够的实用灵敏度和较宽的线性范围。相对于商用荧光检测器，该检测器具有成本低，光学系统结构简单，易于校准和实用性强等特点，适合荧光试剂和染料等产品的日常分析。第3章：高效液相色谱法测定乙二醇中甲醛和乙醛含量。建立了高效液相色谱法测定乙二醇中甲醛、乙醛含量的分析方法。将样品与过量的2,4-二硝基苯肼（DNPH）衍生液混合，样品中的甲醛和乙醛分别与2,4-二硝基苯肼（DNPH）发生衍生反应，生成甲醛、乙醛的苯腙衍生物，直接用高效液相色谱—紫外检测器在360nm的波长下进行检测，应用标准曲线外标方法计算结果，能准确、快速、灵敏地检测乙二醇中的甲醛、乙醛。关键词激光诱导荧光检测器高效液相色谱乙二醇甲醛乙醛AbstractThispaperconsistsofthreechapters.Thefirstchapterisintroduction.Thischaptermainlydescribestheprinciplesoffluorescencedetection,thecompositionofLIFD,structureandcombinedwithotherdetectiontechnologies.Italsointroducetheprospecttothefuture.Thesecondchapteristhatdesignandtestofpracticalandsimplelaser-inducedfluorescencedetector.Basedonorthogonalstructure,developedasimpleHPLClaser-inducedfluorescencedetector.Itused930fluorescencespectrometerfortheskeletonandborrowedShimadzuRF-535testpool.Togetherwithself-adjustablepowersupply,itapplied532nmand405nmwavelengthlaserastheexcitationsourceofselfrespectively,withRhodamineBandFluoresceinasthefluorescentreagentevaluatetheperformanceofthesystem.Testresultsshowthatthedetectormetthedesignrequirements.Ithadenoughpracticalsensitivityandwidelinearrange.RelativetotheCommercialfluorescencedetector,thedetectorhadaverylowcost,opticalsystemissimple,easycalibrationandpracticaladvantages.Itissuitablefortheroutineanalysisthatproductsweresuchasfluorescentreagentsanddye.ThethirdchapteristhatdeterminationofformaldehydeandfcetaldehydeinethyleneglycolbyHPLC-UVD.Theinjectorandtherelativegaspipelinesystermweremodified.Theresearchofaccuracywasdetectedusingmixedalcohol,andtheresultshowthatthereproducibilityandtheaccuracymeetthedesignrequirements.KeywordsLaser-inducedfluorescenceDetectorHPLCethyleneglycolformaldehydeacetald目录第1章绪论...............................................................................