hsp70cd80嵌合dna疫苗通过调节趋化因子及受体对哮喘小鼠气道炎症干预作用及机制的分析-analysis of the intervention effect and mechanism of hsp 70 cd 80 chimeric dna vaccine on airway inflammation in asthmatic mice by regulating chemokines and receptors.docx

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2018-05-29 发布于上海
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hsp70cd80嵌合dna疫苗通过调节趋化因子及受体对哮喘小鼠气道炎症干预作用及机制的分析-analysis of the intervention effect and mechanism of hsp 70 cd 80 chimeric dna vaccine on airway inflammation in asthmatic mice by regulating chemokines and receptors.docx

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hsp70cd80嵌合dna疫苗通过调节趋化因子及受体对哮喘小鼠气道炎症干预作用及机制的分析-analysis of the intervention effect and mechanism of hsp 70 cd 80 chimeric dna vaccine on airway inflammation in asthmatic mice by regulating chemokines and receptors

TheInfluenceofHSP70/CD80DNAVaccineonAirwayInflammationandmechanismofinterventionbyRegulatingtheChemokineandtheReceptorofChemokineinAsthmaticMiceAbstractObjective:Bronchialasthma(asthma)isacomplexchronicinflammatoryairwaydiseases,whichofthemainpathologicalfeaturearesevereairwayinflammation,AHRandairwayremodeling,involvingavarietyofinflammatorymediatorsandimmunecellstotheroleofairway.TlymphocytesaredividedintoTh1andTh2byproducedcytokinestypeoftheThelpercell(Th)cells,andtheTh1/Th2imbalanceisthemostimportantpathogenesisofasthma.InadditiontoCD4+Tcells,thereareothercellsinvolvedintheinflammatoryprocessofasthma,includingeosinophils,basophils,lymphocytes,neutrophils,mastcells,etc,whilethechemokinesplayanimportantroleinthosecellsactivationandmigrationtotheairway.ThisexperimentisintendedtodiscusswhetherthepathogenesisofasthmabyregulatingthechemokineandchemokinereceptortoactontheTh1/Th2balanceinordertoachievethepurposeofthetreatmentofasthma.HSP70/CD80DNAvaccineshowedsignificantinhibitionofmycobacteriumtuberculosisintuberculosisinmice,manifestedasIFN-γsignificantlyhigher,strengthenedofTh1response,inhibitedtoTh2response,reachingthestateoftheTh1/Th2immunebalanceandkillingmycobacteriumtuberculosis.Inthisstudy,weinvestigatethepreventiveeffectofHSP70/CD80DNAvaccineontheairwayinflammationandhyperresponsivenessaswellasTARCanditsreceptorCCR4expressioninthelungtissueinasthma.DiscusstheroleofIL-4、CCR4、TARCinairwayinflammationinasthmaanditsmechanismofselectiveregulationofTh2cells.Methods:ThesizeofHSP70andCD80genesinplasmidwasidentifiedbyPCR,prepareHSP70/CD80DNAplasmidandpVAX1plasmid,detecttheconcentrationandpurityanddissolveitintoNSforfurtheruse.OVA-sensitizedmicetoestablishamousemodelofasthma.40femalehealthyBALB／cmicewererandomlydividedinto4groupsandeverygroupincluded10mice.ThevectorgroupandthevaccinegroupinjectedwithpVAX1plasmisandHSP70/CD80DNAplasmid,andthecontrolgroupinjectedwithsaline.For24hafterthelastOVAstimulateinhaledAchEbytheplethysmographdetectedthelevelofairwayreactivity.Eyebl