ifitm3基因对肝癌细胞侵袭及迁移能力影响-effect of ifitm 3 gene on invasion and migration ability of hepatocellular carcinoma cells.docx

ABSTRACTBackground:Hepatocellular carcinoma (HCC) is one of the highest morbidity malignant tumors worldwide. HCC usually expresses as early vascular invasion, capsular invasion and metastasis inside and outside the liver, although we can take some intervention studies to the recurrence and metastasis of liver cancer on clinic, such as preoperative percutaneous transcatheter arterial embolization (TAE) or chemoembolization (TACE) postoperative TACE and biological treatment interventions, but the overall effect of these measures is still very limited, the key point lies in the molecular mechanisms of HCC recurrence and metastasis is still not entirely clear to us and we realise less knowledge about the molecular regulatorynetworks. therefore ， the study on the liver cancer recurrence and molecularmechanisms and taking effective measures to prevent and treat the medical profession is the major problems need to be solved.Interferon induced transmembrane protein (IFITM) is a discovered antiviral protein family in the cell recently, which inhibit many with or without coated virus’ replication to take effect on antiviral. At the same time, some recent studies show that IFITM3 plays an important role in migration of a variety of human tumor cells and stem cells, but we have not yet realized the liver cancer occurrence, development, diffusion transfer and the mechanism of IFITM3.Purpose:Investigate the biological function of IFITM3 in hepatoma cells.Methods:Develop hepatoma cell lines, using Western-Blot method to detect IFITM3’expres-sion in all hepatoma cell lines and select IFITM3 high expression liver cancer cell lines.We construct expression vectors of specific shRNA for IFITM3 and transfect the expression vectors into hepatoma cell lines of overexpression IFITM3. Screeningthe most effective shRNA interference clips through Western Blot or RT-PCR.We will transfect the most effective shRNA interference clips into IFITM3 high expression of hepatoma cell lines an