igf1对人牙髓细胞增殖分化影响的体外及 裸鼠体内研究-effects of i gf1 on proliferation and differentiation of human dental pulp cells in vitro and in nude mice.docx

目录中文摘要···········································································································1英文摘要···········································································································4英文缩写···········································································································7研究论文IGF-1对人牙髓细胞增殖分化影响的体外及裸鼠体内研究前言···········································································································8材料与方法·······························································································9结果··········································································································17附图··········································································································19附表··········································································································26讨论··········································································································27结论··········································································································32参考文献··································································································33综述支架在牙髓再生中的应用···································································36致谢··················································································································46个人简历··········································································································47IGF-1对人牙髓细胞增殖分化影响的体外及裸鼠体内研究摘要目的：探究在胰岛素样生长因子-1（insulin-likegrowthfactor-1,IGF-1）的作用下，人牙髓细胞体外及裸鼠体内的增殖、分化情况。方法：本实验选取因阻生或者正畸减数拔除的健康恒牙（经患者知情同意），采用组织块酶消化法分离、提取人牙髓细胞并进行原代培养，当细胞增殖达到培养瓶底的80%时，用0.25%胰蛋白酶消化并且传代，选取第四代人牙髓细胞作为种子细胞进行以下实验：1IGF-1对人牙髓细胞体外增殖的影响以2×104个/孔人牙髓细胞接种于96孔培养板中，按照5行×5列，共25个孔的规格进行接种，共接种4个板。细胞培养24小时后，吸净培养液，每一列为一组，实验组分别用含2%血清浓度的50ng/ml、75ng/ml、100ng/mlIGF-1培养液继续培养，对照组用含2%血清浓度的培养液培养，每三天更换一次培养液，分别于第1天、第3天、第5天、第7天采用MTT法检测细胞的体外增殖情况。MTT法检测具体如下：每孔加20μl甲基