il10对子痫前期胎盘氧化应激的影响及其与nox1的相关性-effects of il10 on placental oxidative stress in preeclampsia and its correlation with nox 1.docx

独创性声明本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成 果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或 集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文 中以明确方式标明。本人完全意识到本人将承担本声明引起的一切法律后果。学位论文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有 权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和 借阅。本人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据 库进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。本论文属于保密□ ，在___ 年解密后适用本授权书。不保密□。（请在以上方框内打“√” ）学位论文作者签名：指导教师签名：日期：年月日日期：年月目录摘要································································································· 1 中文摘要·························································································· 1 英文摘要·························································································· 2 前言································································································· 3IL-10 对子痫前期胎盘氧化应激的影响及与 NOX1 的相关性········································································································ 6 材料与方法························································································· 6 结果································································································ 13讨论································································································ 20参考文献·························································································· 24综述······························································································· 28致谢······························································································· 42摘要【目的】探讨IL-10在子痫前期胎盘氧化应激发病机制中的作用及与NOX1的关系。【方法】采集经剖宫产子痫前期患者及同期正常妊娠孕妇胎盘组织各15例为研究对 象。用实时定量PCR(Real-time RT-PCR) 的方法检测胎盘组织IL-10 mRNA、 NOX1 mRNA的表达水平。比色法检测胎盘组织MDA含量。统计比较数据并相关分析。【结果】1. 与正常妊娠组相比较，子痫前期患者胎盘组织IL-10 mRNA表达水平降低（P0.05）。NOX1 mRNA表达水平升高（P0.05）。差异有统计学意义。 2．与正常妊娠组相比较，子痫前期患者胎盘组织MDA含量明显升高（P0.01）。3. 相关分析显示子痫前期患者胎盘组织IL-10 mRNA表达水平与MDA含量呈负 相关（P0.05），与NOX1 mRNA表达水平呈负相关（P0.01）。【结论】子痫前期患者胎盘组织氧化应激水平升高，与 IL-10 及 NOX1 表达水平密切 相关。IL-10 减轻胎盘氧化应激的途径可能有多种，降低 NOX1 的表达水平可能 是其中