il15在鼻息肉发病中作用的初步分析-preliminary analysis of the role of il15 in the pathogenesis of nasal polyps.docx

含量。2. 用免疫组化法检测其组织中 Bcl-2 及 Bcl-xl 的表达情况。3. 用原位杂交方法检测组织中 IL-15mRNA 的表达情况。结果1. 鼻息肉组中 IL-15 蛋白表达高于下鼻甲组，差别有统计学意义（P0.05）。2. 鼻息肉组中 Bcl-xl 表达高于下鼻甲组，表达主要位于粘膜下固有层下浸润的以 EOS 为主的炎症细胞，差别有统计学意义(p0.01)；Bcl-2 的表达主要位于上皮，但两组 间差别无显著性；IL-15 蛋白的表达与 Bcl-2 和 Bcl-xl 的表达之间没有相关性。3. 鼻息肉组中 IL-15mRNA 在粘膜下固有层浸润的以 EOS 为主的炎症细胞有很强的表 达，与下鼻甲组 IL-15mRNA 表达的差别有统计学意义（p0.05）。IL-15mRNA 在多数鼻息肉 中的粘膜下腺体上皮细胞有表达，部分粘膜上皮细胞有表达，以上的表达都强于下鼻甲组 的表达。粘膜下固有层炎症细胞 IL-15mRNA 的表达与 Bcl-xl 的表达有相关性（r＝0.592）。结论1. IL-15 通过旁分泌或自分泌的方式作用于位于粘膜下固有层中以嗜酸性粒细胞为 主的炎症细胞，并通过 Bcl-xl 途径诱导的凋亡抑制在鼻息肉的发病中很可能起着重要的 作用。2. IL-15 在鼻息肉中粘膜下腺体的表达强于在下鼻甲中的表达。可能参与了鼻息肉形 成过程中粘膜下腺体分泌功能紊乱的病理过程，但其机制尚不明确。3. IL-15 可能有通过诱导 Bcl-2 表达而抑制鼻息肉粘膜上皮细胞凋亡的作用。关键词白细胞介素-15；鼻息肉；嗜酸性粒细胞；凋亡；炎症AbstractBackground and ObjectNasal polyp is a disease of chronic nasal mucous membrane-nasal sinuses mucous membrane inflammation, as a very common disease of human respiratory tract, it is a very severe threaten to human health and bring down patient’s standard of living. A lot of articles reported the pathogenesis of chronic nasal mucous membrane - nasal sinuses mucous membrane inflammation is very complicated and unclear, so exact etiology and pathogenesis of nasal polyp are still under debate. In the past 10 years, the role of cytokines in the pathogenesis of nasal polyp are very arrestive. Recently, A lot of article reported the pathogenesis of nasal polyp is a overbalancing course of cell proliferation and apoptosis, and some researchers reported the abnormal apoptosis of eosinophil along with a lot of cytokines’s abnormal expression in nasal polyp. So, regulate the chronic inflammation of nasal polyp by modulate eosinophil apoptosis has became a new field to discover nasal polyp’s pathogenesis.IL-15, after it’s discovery byGrabstein in 1994, lot of research indicated it has a similar bioactivity with IL-2, both of them play a important role in inflammation. A very important bioactivity of it is the function of restrain apoptosis of inflammatory cells, such as eosinophil, lymphocyte andma