精神分裂症小鼠脑内gaba能神经元的变化-changes of gaba neurons in schizophrenic mice brain.docxVIP

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精神分裂症小鼠脑内gaba能神经元的变化-changes of gaba neurons in schizophrenic mice brain.docx

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精神分裂症小鼠脑内gaba能神经元的变化-changes of gaba neurons in schizophrenic mice brain

The change of GABAergic neurons in the mouse brain of schizophreniaABSTRACTObjective In this study, observed the stereotyped and ataxia behavior, and the changes in the prefrontal cortex (PFC) and hippocampus (HIP) CA1, CA2, and CA3 within of GABA in models of schizophrenia mouse.Methods Experiments were carried out on C57BL/6 mice and GAD67-GFP knock in mice. Animals were injected with NMDAR antagonist MK801 (Dizocilpine) (0.3 mg/kg, 0.6 mg/kg, 1 mg/kg, respectively) for two weeks to induce schizophrenia. To examine the stereotyped movement, we have scored for stereotyped movement using scales on 3, 5, 7, 10 and 14 d after MK801 injecton. Using immunofluorescence methods, the morphology ofGABAergic subpopulation markers, calcium-binding protein(CB) 、 calretinin (CR) 、parvalbumin (PV) were observed, and RT-PCR were used to examine the mRNA level.Result(1) behavior change.○1Stereotyped behavior assessment will be 0.3 mg/kg,0.6 mg/kg, 1 mg/kg three doses of mouse stereotyped behavior scale score compared. The results showed that, 0.6 mg/kg, 1 mg/kg doses of mouse stereotyped behavior score is significantly higher than that of 0.3 mg/kg dose group, respectively 37.9%, 38.1%, and 0.6 mg/kg doses groups and 1 mg/kg doses no obvious difference between groups score; After the treatment of 3, 5, 7, 10 and 14 days, along with the increase of drugs for days, each dosegroup of stereotyped behavior score were in mouse reduced gradually. ○2 Ataxia assessmentwill be 0.3 mg/kg, 0.6 mg/kg, 1 mg/kg three doses of mouse ataxia scale score compared, the results showed that 1 mg/kg doses of mouse stereotyped behavior score significantly higher than 0.3 mg/kg dose group, increased 36.4%. It is no obvious difference between 0.3 mg/kgdoses group and 0.6 mg/kg doses group, 1 mg/kg doses group and 0.6 mg/kg doses groups score; After the treatment of 3, 5, 7, 10 and 14 days, with the increase of drugs for days, each dose group of mouse ataxia scores were gradually reduced in mouse. (