竞争条件下环境因子对铜绿微囊藻影响的分析-analysis of the influence of environmental factors on microcystis aeruginosa under competitive conditions.docxVIP

平 4（8.0×105cells/mL）在 BG-11 培养液，温度 25℃，光照强度为 7500Lx 下按不 同丰度梯度做随机实验。测不同实验组的铜绿微囊藻细胞数，所得数据求其生长速率、 拐点时间及最大生物量，取生长速率最小，拐点时间最大的实验组。结果表明 ABCE 组，即铜绿微囊藻、淡水小球藻、四尾栅藻、鱼腥藻混养组，对铜绿微囊藻的抑制效 果最为明显。最后，在相同的实验条件下，取铜绿微囊藻、淡水小球藻、四尾栅藻、鱼腥藻四 种微藻，按照以上实验所得出的起始密度组合，分别设置五种光照强度水平（500LX、 2500LX、5000LX、10000LX、15000LX）和四种温度水平(15℃、25℃、30℃、35℃)。 结果显示，竞争条件下微囊藻的最大生物量均小于其单独培养的生物量，竞争条件下 铜绿微囊藻在光照强度为 10000LX 和温度为 35℃时取得最大生物量，而在 500Lx 和 15℃时微囊藻生长受到明显抑制，整个实验阶段生物量变化不大。不同的光照强度和 温度对铜绿微囊藻生长状况的影响均存在显著差异（P0.05），各实验组竞争条件下 铜绿微囊藻的生长状况与单独培养存在显著的差异（P0.05）。关键词：微型藻，起始密度，铜绿微囊藻，竞争抑制参数，光照强度，温度，最 大生物量AbstractOn the suitable common condition for training, temperature 25 ℃ , light intensity 7500 Lx, the nutrient formula BG-11, and researching the different starting density, micro by group of abundance, light intensity, temperature and other factors have an affect on the growth of M.aeruginosa which is a familiar advantage kind in the pond water hua .Theresults showed that different micro algae and micro by group of abundance have a remarkable differences impact on the growth of M.aeruginosa of (P 0.05); The micro algae growth situation between different starting density are also significant difference (P 0.05); And under the same training the micro by group of abundance and starting density have interaction effect of the M.aeruginosa; After study, the growth of M.aeruginosa under the competition condition have a significant different in different light intensity and the temperature (P 0.05). Finally, make a conclusion abut the micro algae and environmental factors of the micro algae characteristics which is the effect factors on the growth of M.aeruginosa in freshwater aquaculture ponds, for the probe of the freshwater pond environment for regulation of micro algae composition, the method of the replacement to provide the theory basis.In this paper, comparing with the Micro algae growth under separate training in the same period, five common microalgae in freshwater pond, such as C.vulgaris, S.quadricauda-FACHB-1297, K.luna