辽宁沿海蛤仔群体种质资源调查分析-investigation and analysis on germplasm resources of clam populations in liaoning coastal areas.docxVIP

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辽宁沿海蛤仔群体种质资源调查分析-investigation and analysis on germplasm resources of clam populations in liaoning coastal areas.docx

辽宁沿海蛤仔群体种质资源调查分析-investigation and analysis on germplasm resources of clam populations in liaoning coastal areas

wildR.philippinarumpopulationstoassessthegeneticdiversityandpopulationdifferentiation.Atotalof114allelesweredetectedon12SSRloci,andthenumberofallelesperlocusineachpopulationrangedfrom2to11,andallelicrichnessperlocusvariedfrom2.00to10.88foreachsample.Theaverageofobservedandexpectedheterozygosityrangedfrom0.386to0.550,andfrom0.533to0.707,respectively.PairwiseFSTvaluesindicatedthatallpopulationpairshadsignificantgeneticdifferentiation(overallFST=0.242,P0.05).ClusteranalysisusingUPGMAseparatedtheeightpopulationsintotwogroups.ThisstudywillshedlightonthedomesticationandcultivationonpopulationgeneticdiversityofR.philippinarum,andalsoprovidethefoundationforconservationofR.philippinarumgermplasmresourcesinclambreedingpractice.Keywords:Ruditapesphilippinarum;SSRmarkers;germplasmresources;geneticdiversity目录摘要...........................................................................................................................................IAbstract.......................................................................................................................................II目录..........................................................................................................................................IV第一章绪论.................................................................................................................................11.1遗传多样性及其研究方法............................................................................................11.1.1遗传多样性的定义...................................................................................................11.1.2遗传多样性的研究方法...........................................................................................形态学标记..........................................................................................................................细胞学标记..........................................................................................................................生化标记.............................................

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辽宁沿海蛤仔群体种质资源调查分析-investigation and analysis on germplasm resources of clam populations in liaoning coastal areas.docxVIP