六种石斛多糖对酒精性肝损伤干预作用的比较分析-comparative analysis of intervention effects of six dendrobium polysaccharides on alcoholic liver injury.docxVIP

ComparisonofinterventioneffectsofpolysaccharidesfromsixDendrobiumspeciesonalcoholicliverinjuryABSTRACTFirstly,thecontentsoftotalcarbohydrate,totalnitrogen,solubleproteinanduronicacid,intrinsicviscositiesandthemonosaccharideprofilesofsixpolysaccharidesincludingDHP,DOP,DFP,DCP,DNPandDMP,obtainedbywaterextractionandethanolsedimentationfromtheplantsofDendrobiumhuoshanense,D.officinale,D.fimbriatum,D.chrysotoxum,D.nobileandD.moniliforme,respectively,wereanalyzed.ThentheeffectsofdifferentdosesofsixDendrobiumpolysaccharidesonweightgain,liverindex,biochemicalindicatorsinserumandlivertissues,activitiesofantioxidantenzymesandalcoholmetabolizingenzymesandtheexpressionofcytokinesbyreplicatingthemodelofalcohol-inducedliverinjuryinmicewereevaluated.Theresultsshowedthatthecontentsoftotalcarbohydrate,totalnitrogen,solubleproteinanduronicacidofthesixDendrobiumpolysaccharideswereintherangeof74.27%?93.10%,1.60%?3.68%,0.15%?0.80%and3.92%?10.90%,respectively;andthehighestcontentsofwhichwereDOP,DHP,DFP,DNPandDFP,respectively;whilethelowestcontentsofwhichwereDNP,DCP,DCP,DMPandDHP,respectively.TheintrinsicviscositiesofthesixDendrobiumpolysaccharideswereintherangeof35.05?134.30cm3/g.DFPhadthehighestintrinsicviscosityandDHPhadthelowestone.Monosaccharideanalysisshowedthatallthepolysaccharidesweredeterminedtobeheteropolysaccharidescontainingmannose,galactoseandglucoseasmajorsugarandtracesofothermonosaccharide.TheresultsofexperimentontheinterventioneffectsofDendrobiumpolysaccharidesonalcohol-inducedliverinjuryinmiceindicatedthatthelevelsofALT,AST,ALP,TBIL,TC,TGandLDL-CinserumandTC,TGandMDAinlivertissueshadsignificantlyincreased,whilethelevelsofADH,ALDH,SOD,CAT,GSH-Px,GR,GSTandGSHinlivertissues,aswellasHDL-Cinserum,hadsignificantlydecreasedinalcoholcontrolgroupmice,whencomparedtothenormalcontrolgroup.TheDendrobiumpolysaccharidesplayedanefficientroleintherestorationofthealcohol-inducedliverinjury,whichwereevidencedbythefollowing:①ThedisturbedlevelsofserumALT,AST,ALPandTBILinmicewithalcohol-induce