HPLC法和分光光度法测定茴三硫片含量比较.docVIP

HPLC法和分光光度法测定茴三硫片含量比较 　　[摘要] 目的：运用高效液相色谱法和紫外-可见分光光度法对茴三硫片含量进行测定，并对方法学和测定结果进行比较。方法：色谱法中色谱柱Kromasil C18柱(4.6 mm ×250 mm，5 μm)，以甲醇-水（80∶20）为流动相，检测波长为432 nm；分光光度法于432 nm波长处以外标法测定。结果：HPLC法在32.5～250.0 μg/ml浓度范围内其浓度与峰面积呈良好线性关系（r=0.999 9），平均空白回收率为99.68%（RSD=0.46%，n=9）；分光光度法在4.096～20.480 μg/ml浓度范围内其浓度与吸光度呈良好线性关系（r=0.999 4），平均回收率为99.96%（RSD=0.44%，n=9）；三批样品的平均含量测定结果为：HPLC法分别为99.25%、99.14%和99.10%，分光光度法分别为99.46%、99.22%和99.13%。结论：两种方法均能准确测定茴三硫片的含量，可作为其质量控制的方法。 　　[关键词] 茴三硫片；HPLC；分光光度法；含量 　　[中图分类号] R917 [文献标识码] A [文章编号] 1673-7210（2011）07（a）-057-03 　　 　　Comparison of the content assay of Anetholetrithione Tablets determined by HPLC and visible spectrophotometry 　　WANG Jialong1, LIU Congbin2 　　1.Pharmacy Department Office, Peoples Hospital of Shucheng, Shucheng 231300, China; 2. School of Pharmacy, Anhui College of Traditional Chinese Medicine, Hefei 230038, China 　　[Abstract] Objective: To compare the HPLC and visible spectrophotometry methods in assaying Anetholetrithione Tablets, and the methodology and results were compared respectively. Methods: Kromasil C18 (4.6 mm×250 mm,5 μm) was used with mobile phase of methanol-water (80∶20) and the detective wavelength was set at 432 nm in HPLC method; the detective wavelength was set at 432 nm in visible spectrophotometry method. Results: The linear range of anetholtrithione was 32.5-250.0 μg/ml (r=0.999 9), and the average recovery was 99.68% with RSD 0.46%(n=9) in HPLC method; the visible spectrophotometry method was 4.096-20.480 μg/ml (r=0.999 4), and the average recovery was 99.96% with RSD 0.44%(n=9). The average assay results of three batches of samples: HPLC method was 99.25%, 99.14% and 99.10%, visible spectrophotometry method was 99.46%,99.22% and 99.13%. Conclusion: Both methods can accurately determine Anetholetrithione Tablets, and can be used as the quality control methods of it. 　　[Key words] Anetholetrithione Tablets; HPLC; Visible spectrophotometry; Content determination 　　 　　茴三硫又名胆维他，其药理作用广泛，具有保护肝细胞、利胆、抗氧化、降低