“面口合谷收”形态学基础.docVIP

“面口合谷收”形态学基础 　　[摘 要] 目的：探讨合谷穴与口面部联系的形态学基础。方法：采用荧光素单标记和双标记法，将荧光素碘化丙啶(PI)和双苯甲亚胺(Bb)分别注入“合谷”穴区和“四白”穴区，取同侧脊神经节颈1～颈8，胸1～胸2，颈上、中、下交感节及三叉神经半月节，荧光显微镜下观察计数。结果：在脊神经节颈5～颈8观察到大量PI单标记细胞，主要分布于颈6～颈7，在三叉神经半月节也可见大量Bb单标记细胞，另外在三叉神经半月节可见少量PI～Bb双标记细胞，占标记细胞的4.5%。结论：三叉神经半月节有向“合谷”穴和“四白”穴 的分支投射，这可能是“面口合谷收”的形态学基础。[主题词] 荧光双标术；穴，合谷；口颌系统/神经支配；神经节，脊 Study on morphological foundation of point LI 4 treating disease s in the face and mouth Jing Xianghong, Cai Hong, Lu Bo, et al. (Institute of Acupunc ture Moxibustion, China Academy of TCM, Beijing 100700, China) ABSTRACT Objective To study on morphological link of Hegu (LI 4) with the face and mouth. Methods Fluorescein single-label and double-label methods were used, fluorescein propidium iodide (PI) and bi-benzylmethy benzhy dryl imine (Bb) were respectively injected into Hegu (LI 4) point area and Sibai (ST 2) point area. Ipsilateral spinal ganglia C1-C8，T1-T2, superior cervical ganglion (SCG), middle cervical ganglion (MCG), inferior cervical gangl ion (ICG) and Gasserian ganglion were taken for count of the cells under fluores cence microscope. Results A lumber of PI single-labeled cells w ere observed in C5-C8 and the Gasserian ganglion, mainly in C6-C7, and aless PI-Bb double-labelled cells, accounting for 4.5% were found in the Ga s serian ganglion. Conclusion The Gasserian ganglion has branchin g projection to Hegu (LI 4) and Sibai (ST 2). This is possibly morphological fou ndation of Hegu treating diseases in the face and mouth. KEY WORDS Fluorescent Labeling; Point, LI 4 (Hegu); Stomatognat hic System/innerv; Ganglia, Spinal 　　 　　合谷穴为手阳明大肠经的原穴，位于第1、第2掌骨之间，约当第2掌骨桡侧之中点，具有祛风散邪、清宣阳明热邪和清泻头面诸窍热邪之效。其临床应用广泛，用于头面五官疾病的治疗，如头痛、三叉神经痛、颜面肿痛、目赤肿痛、牙痛、耳鸣、耳聋、下颌关节炎、面神经麻痹等均有明显疗效。“合谷”穴用于治疗这么多种疾病，完成如此复杂功能，其形态学基础是什么?为此我们采用荧光素双标记法探讨了合谷穴的传入途径。1 材料与方法1.1 材料与注射方法 　　健康成年家猫10只，体重3～5 kg，雌雄不拘。戊巴比妥钠(20 mg/kg)腹腔麻醉后，按照比较神经解剖学取穴，用微量注射器将1%碘化丙啶(Propidium Iodide, PI)10 ?缓慢注入猫“合谷”穴区(位于前爪一、二掌骨之间)，动物存活72 h。再次麻醉后，将1%双苯甲亚胺(Bb)10注入同侧“四白”穴区(眶下神经处)，动物存活12～16 h。再次麻醉，经升主动脉依次灌注4