墨西哥仙人掌多糖对人乳腺癌MCF-7细胞凋亡坏死诱导作用研究.docVIP

墨西哥仙人掌多糖对人乳腺癌MCF-7细胞凋亡坏死诱导作用研究 　　[摘要] 目的 观察墨西哥仙人掌多糖对人乳腺癌MCF-7细胞凋亡及坏死的诱导作用。方法 将浓度为20μg/mL的墨西哥仙人掌多糖作用于体外培养的人乳腺癌MCF-7细胞，分别培养24h和48h，然后利用流式细胞仪检测人乳腺癌MCF-7细胞的凋亡及坏死情况。结果 不同作用时间的墨西哥仙人掌多糖均能够诱导人乳腺癌MCF-7细胞凋亡，并促进该肿瘤细胞发生坏死，与阴性对照组比较差异显著（P＜0.01），尤以48h作用明显，凋亡及坏死肿瘤细胞占培养细胞总数的84.11%。结论 墨西哥仙人掌多糖能够诱导人乳腺癌MCF-7细胞的凋亡及坏死。 　　[关键词] 墨西哥仙人掌多糖；凋亡；坏死 　　[中图分类号] R737.9 [文献标识码] A [文章编号] 1673-9701（2011）23-14-02 　　 　　Studies on the Effects of Mexican Cactus Polysaccharides in Inducing Apoptosis and Necrosis of Human Breast Cancer MCF-7 Cells 　　JI Hui TIAN Hua WANG Yuchun DONG Haiying MIAO Shu WANG Yu LI Tao 　　Qiqihar Medical College, Qiqihar 161006, China 　　 　　[Abstract] Objective To observe the effects of Mexican cactus polysaccharides in inducing apoptosis and necrosis of human breast cancer MCF-7 cells. Methods The human breast cancer cells cultivated in vitro were treated with 20μg/mL of polysaccharides of Mexican cactus. The effects of Mexican cactus polysaccharides in inducing apoptosis and necrosis of human breast cancer MCF-7 cells were determined by flow cytometry. Results Compared with negative control group, polysaccharides of Mexican cactus can significantly lead to the apoptosis and necrosis of human breast cancer MCF-7 cells in both 24 hours group and 48 hours group, especially the 48 hours group which total percentage of apoptosis and necrosis was 84.11% in the cultivated cells. Conclusion Polysaccharides of Mexican cactus can induce the apoptosis and necrosis of human breast cancer MCF-7 cells. 　　[Key words] Polysaccharides of Mexican cactus; Apoptosis; Necrosis 　　 　　乳腺癌是我国危害女性健康发病率最高的恶性肿瘤，其治疗方法一直是人们关注的焦点[1]。我们的前期实验以及其他研究结果表明，墨西哥仙人掌多糖可对人胃腺癌SGC-7901细胞、大肠癌Lovo细胞、肺腺癌Anip细胞、宫颈癌Hela细胞等多种肿瘤细胞增殖有明显抑制作用[2，3]，故今将墨西哥仙人掌???糖作用于体外培养的人乳腺癌MCF-7细胞，通过流式细胞仪检测技术来判定墨西哥仙人掌多糖是否能够诱导人乳腺癌MCF-7细胞的凋亡及坏死，以期为乳腺癌患者找到更合理、有效的治疗方法。 　　1 材料与方法 　　1.1 材料 　　1.1.1 人乳腺癌MCF-7细胞 齐齐哈尔医学院基础医学研究室惠赠，常规培养于含10%小牛血清的RPMI-1640培养液中。 　　1.1.2 药物及试剂 墨西哥仙人掌多糖由齐齐哈尔大学提供；Annexin Ⅴ-FITC/PI凋亡试剂盒及小牛血清购自哈尔滨宝泰克生物科技有限公司；RPMI-1640培