皮肤交感物质分布线发现及其与中医经络实质关系.docVIP

皮肤交感物质分布线发现及其与中医经络实质关系 　　[摘 要]目的：为了深入研究非血管性经络的实质――皮肤交感神经敏感线或皮肤交感物质富集线。方法：以125I-酪氨酸为示踪剂，用宏观放射自显影的方法。结果：显示出大鼠皮肤中存在纵贯全身的系列交感物质分布线，连续清晰，左右对称，在头部和肢体末端形成环路。在沿物质分布线经过的背上部切断皮肤，可以显着阻断针刺“足三里”产生的针刺效应，表明这些交感物质分布线就是针刺信号的传递线。结论：皮肤交感神经敏感线的假说得到了形态学的证明，或者说经络的形态学基础已经显现出来。 　　[主题词]经络实质；针刺镇痛；信号传递；皮肤/化学；肾上腺皮质激素类/分析 　　Development of sympathetic substance lines on the skin and its relationship with meridian essence 　　Liu Liyuan, Zhang Hui, Pan Juan (College of Life Science, Bei jing Normal University, Beijing 100875, China) 　　ABSTRACT Objective To probe deeply into the essence of non-blood vessel meridians――sympathetic sensitive lines or sympathetic substance-rich lines on the skin.Methods Macro-autoradiography was used with 125 I-tyrosine as tracer in the rat. 　　Results A series of sympathetic substance lines passed through the skin of the whole body were displayed, which were continuous, clear, and sym metric, and connected each other in the head and the end of limbs to form loops. When the skin on the upper of the back where the sympathetic substance lines p a ssed was cut, the effect of acupuncture at Zusanli (ST 36) was significantly blo cked, indicating that the sympathetic substance lines on the skin are conduction pathway of the acupuncture signal. Conclusion It is proved by morphology that the essence of merid ians is sympathetic substance lines. 　　KEY WORDS Meridian Essence; Acupuncture Analgesia； Skin/chem; Signal Transduction; Adrenal Cortex Hormones/anal 　　 　　笔者在动物的针刺镇痛模型上，对皮肤中是否存在特定的信号传递通路及通路的物质基础进行了系列的研究。由于切断大鼠针刺穴位上下的皮肤可以完全阻断针刺镇痛效应[1]，提示在皮肤中存在针刺信号传递的特定线路；大鼠针刺后经线皮肤中有NA释放出来[2]；在家兔针刺穴位上下的经线皮肤真皮内注射微量的受а体阻断剂可以阻断针刺镇痛效应[3]；大鼠??位注射微量的а1受体兴奋剂可以模拟很强的镇痛效应[4]，表明皮肤的针刺信号传递线路与交感肾上腺素能物质有密切的关系，这些交感物质(此处及以下所称皆指交感肾上腺素能物质)的释放和对局部а受体的作用是针刺信号传递的核心。由此推测针刺信号的皮肤传递线路上应该有交感物质的优势分布，从形态上称之为皮肤交感物质富集线或从功能上称之为交感神经敏感线[5]。皮肤中是否真的存在交感物质的规律性分布或线性分布呢?为此进行了大鼠皮肤交感物质分布的宏观放射自显影观察，在大鼠皮肤中显示出了连续清晰的交感物质分布线，从头到足，纵贯全身。为了验证这些路线是否就是传递针刺信号的线路，笔者进一步观察了高位切断交感物质分布线对针刺镇痛效应的影响。 　　 　　1 材料与方法 　　 　　1.1 宏观放射自显影法显示交感物质