皮肤鳞状细胞癌及Bowen病中PGP9.5和CyclinD1表达及临床意义.docVIP

皮肤鳞状细胞癌及Bowen病中PGP9.5和CyclinD1表达及临床意义 　　[摘要]目的：探讨 PGP9.5和CyclinD1在皮肤鳞状细胞癌(CSCC)、Bowen病中的表达及临床意义。方法：用免疫组织化学SP法检测22例CSCC，31例Bowen病及10例正常皮肤组织中PGP9.5和CyclinD1的表达情况。结果：PGP9.5与CyclinD1在CSCC的表达明显高于正常皮肤组（P=0.006，P=0.00057， 　　[关键词]皮肤鳞状细胞癌(CSCC)；Bowen病；PGP9.5；CyclinD1；免疫组织化学 　　[中图分类号]Q813.1 [文献标识码]A [文章编号]1008-6455（2012）03-0424-03 　　 　　Expression and Significance of PGP9.5 and CyclinD1 in Human Skin Squamous Cell Carcinoma and Bowens Disease 　　NIE Jian-jun1,2,ZENG wei-hui1,GENG song-mei1,XU-lei2 　　(1.Department of Dermatology,The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004,Shaanxi,China; 2.Department of Dermatology of The Second Hospital of Chengdu ) 　　 　　Abstract: Objective To study the expression of PGP9.5and CyclinD1 in Cutaneous squamous cell carcinoma and Bowens disease and exploring the relationship between them and to confirm their role in development of the tumor. Methods Immunohistochemical S-P method was used to examine the expressions of PGP9.5 and CyclinD1 in 22 cases of CSCC, 31cases of Bowens disease, and 10 cases of normal skin. Results The expression of PGP9.5 and CyclinD1 was significantly higher in CSCC than in normal tissue and Bowens disease （P=0.006,P=0.0005720%）。 　　1.4 统计学方法：本实验采用半定量等级资料，观察阳性细胞数，得出表达阳性级别，然后进行等级资料统计，采用非参数等级资料的秩和检验。应用SPSS13.0统计软件，对于计数资料采用确切概率法进行检验，并计算Pearson列联系数判定关联程度。P0.05。PGP9.5在二者之间表达无显著差异。PGP9.5在正常皮肤、CSCC的阳性表达差异比较P=0.006 　　CyclinD1在正常皮肤组织的细胞核表达呈阴性，在10例正常皮肤标本中，有1例弱阳性（阳性率10%）。在Bowen病中，CyclinD1在异型的角质形成细胞细胞核染色阳性，呈黄褐色（图4）。在31例Bowen病例标本中，有15例染色阳性，（阳性率48.4%）。在CSCC中，CyclinD1在鳞癌细胞的细胞核表达呈棕褐色（图5、6）。在22例CSCC例标本中，有17例呈阳性染色（阳性率77.3%）。CyclinD1在正常皮肤与Bowen病阳性表达比较χ2=4.82，P 　　[5]Campbell LK, Thomas JR, Lamp sLW, et al. Protein gene product9. 5 ( PGP 9.5) is not a specific marker of neural and nerve sheath tumors: An immunohistochemical study of 9.5 mesenchymal neoplasm[J]. Mod Pathol,2003,16(10): 963-969. 　　[6]Vujani GM, Kelsey A, Perlman EJ, et al. Anap lastic sarcoma of the kidney: a clin