纯中药制剂“卡宁”对肝癌细胞作用.docVIP

纯中药制剂“卡宁”对肝癌细胞作用 　　[摘要] 目的：观察抗癌中药“卡宁”对体外培养的肝癌细胞株BEL-7402细胞的凋亡抑制基因bcl-2的影响，探讨其抗肿瘤治疗的作用机制。方法：取体外培养的对数生长期BEL-7402细胞，随机分为4组：对照组，5-氟尿嘧啶组，含药“卡宁”血清1组（10%含药血清组）和含药“卡宁”血清2组（15%含药血清组）；采用MTT比色法观察不同浓度含药血清对体外培养的人肝癌细胞株BEL-7402细胞的抑制能力，PCR检测其对凋亡抑制基因bcl-2的影响。结果：PCR结果显示，“卡宁”1、2组bcl-2的活性明显低于对照组(P=0.038)；MTT结果显示，细胞生长抑制率随含药血清作用时间及浓度的增加而增大(P 　　[关键词] bcl-2；中草药；肝脏肿瘤 　　[中图分类号] R735.7[文献标识码]A [文章编号]1673-7210（2009）01（a）-014-03 　　 　　Effect of chinese medicine Caning on hepatic cancer cells 　　MAO Xiao-ling, PAN Su-ying, ZHANG Da-peng 　　(The First Affiliated Hospital of Guangzhou Medical College, Guangzhou510120, China) 　　[Abstract] Objective: To observe the effects of anticancer chinese medicine Caning on the anti-apoptosis gene bcl-2 of in vitro cultured human hepatic cancer cell line BEL-7402 cells, and investigates its anticancer therapeutic mechanism. Methods: In vitro cultured BEL-7402 cells in a logarithmic growth phase were randomly divided into 4 groups: control group, 5-Fluorouracil treated group, serumgroup 1 containing Caning (10% pastille serum) and serumgroup 2 containing Caning(15% pastille serum); then MTT assay was employed to detect the inhibitory ability of serums containing different concentrations of Caning to in vitro cultured human hepatic cancer cell line BEL-7402 cells, and PCR was used to detect the effect of Caning on the anti-apoptosis gene bcl-2. Results: Results of in vitro experiment suggested that, the bcl-2 gene activity in serum group 1 and 2 containing Caning was significantly lower than that of control group (P=0.038); result of MTT assay indicated that the cell growth inhibitory rate was increased along with the increase of the action time and concentration of serum containing Caning(P 本文为全文原貌 未安装PDF浏览器用户请先下载安装 原版全文 　　1.2.2 体外细胞抑制实验――MTT比色法用含0.25%胰蛋白酶和0.02% EDTA的消化液消化对数生长期的BEL-7402细胞，用含20%胎牛血清的DMEM培养基调成2.5×104/ml的单细胞悬液，96孔细胞培养板中每孔加入100 μl，待细胞贴壁6 h后加入含药血清，终浓度分别为10%及15%。每一浓度设3个复孔，并设对照组。分别培养24、48和72 h后，每孔加入0.5% MTT 20 μl，继续培养4 h，1 000 r/min